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ascorbic acid + levan
ascorbic acid 2-fructoside + ?
-
transfructosylation. When 35% of ascorbic acid and 2% of levan are incubated with LFTase of 0.5 unit/g levan at 37°C for 85 h, a maximum 52 g/l of ascorbic acid 2-fructoside is produced
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
levan
di-D-fructofuranose-2,6':2',6 dianhydride + ?
levan
di-D-fructose-2,6':2',6 dianhydride + fructose + limited levan
phlein
di-D-fructose-2,6':2',6 dianhydride
-
while it degrades Phleum pratense phlein more than 90%, formation of 6% reducing sugars as D-fructose
and two or three minor fructooligosaccharides
?
raffinose + D-galactopyranose
?
stachyose + D-galactopyranose
?
sucrose + D-galactopyranose
?
additional information
?
-
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
levan from Zymomonas mobilis: conversion yield 28.4% after 24 h. Levan from Serratia: conversion yield: 28.4% after 24 h
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
levan from Zymomonas mobilis: conversion yield 28.4% after 24 h. Levan from Serratia: conversion yield: 28.4% after 24 h
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
di-D-fructose-2,6':2',6 dianhydride is the main product, 75%. Fructose, levanbiose, and two unidentified oligosaccharides are minor products
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
the enzyme is specifically induced by levan
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
di-D-fructose-2,6':2',6 dianhydride is the main product, 75%. Fructose, levanbiose, and two unidentified oligosaccharides are minor products
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
the enzyme is specifically induced by levan
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
the enzyme degrades Bacillus mesentericus levan up to 40% leaving the levan fragment at the limit of the enzymatic degradation, formation of 5% reducing sugars as D-fructose
and two or three minor fructooligosaccharides
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
levan from Zymomonas mobilis, conversion yield is 35%
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
levan from Zymomonas mobilis, conversion yield is 35%
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
-
-
?
levan
5 di-D-fructose-2,6':2',6 dianhydride + ?
-
-
-
-
?
levan
di-D-fructofuranose-2,6':2',6 dianhydride + ?
-
-
and small amounts of several oligosaccharides and free fructose
?
levan
di-D-fructofuranose-2,6':2',6 dianhydride + ?
-
-
and small amounts of several oligosaccharides and free fructose
?
levan
di-D-fructose-2,6':2',6 dianhydride + fructose + limited levan
-
-
di-D-fructose-2,6':2',6 dianhydride (49.1%) + fructose (3.5%) + limited levan (16.2%)
-
?
levan
di-D-fructose-2,6':2',6 dianhydride + fructose + limited levan
-
-
di-D-fructose-2,6':2',6 dianhydride (49.1%) + fructose (3.5%) + limited levan (16.2%)
-
?
raffinose + D-galactopyranose
?
-
transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside
-
-
r
raffinose + D-galactopyranose
?
-
transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside
-
-
r
stachyose + D-galactopyranose
?
-
transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside
-
-
r
stachyose + D-galactopyranose
?
-
transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside
-
-
r
sucrose + D-galactopyranose
?
-
transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside
-
-
r
sucrose + D-galactopyranose
?
-
transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside
-
-
r
additional information
?
-
-
no activity with dextran, soluble starch, inulin, maltose, sucrose, raffinose, 1-kestose, nystose and 1F-fructofuranosyl nystose
-
?
additional information
?
-
the enzyme never hydrolyzes beta-2,1-fructosyl-linked fructans, such as inulin, as well as oligosaccharides such as 1-kestose and nystose
-
?
additional information
?
-
-
no activity with dextran, soluble starch, inulin, maltose, sucrose, raffinose, 1-kestose, nystose and 1F-fructofuranosyl nystose
-
?
additional information
?
-
the enzyme never hydrolyzes beta-2,1-fructosyl-linked fructans, such as inulin, as well as oligosaccharides such as 1-kestose and nystose
-
?
additional information
?
-
-
no formation of fructose, levanbiose, nor difructose anhydride IV from bifurcose and [O-beta-D-Fru(f)-(2-1)]-O-[beta-D-Fru(f)-(2-6)]2-O-beta-D-Fru(f)-(2-1)-alpha-D-G(p)
-
?
additional information
?
-
-
no activity with inulin, levanbiose, sucrose, dextran or starch
-
?
additional information
?
-
-
no activity with inulin, levanbiose, sucrose, dextran or starch
-
?
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biotechnology
-
production of Arthrobacter levan fructotransferase from recombinant Escherichia coli at high levels via secretion directed by a novel N-terminal motif, TMITNSSSVP. A large amount of extracellular recombinant levan fructotransferase can be produced with high productivity through cost-effective processes
biotechnology
-
production of Arthrobacter levan fructotransferase from recombinant Escherichia coli at high levels via secretion directed by a novel N-terminal motif, TMITNSSSVP. A large amount of extracellular recombinant levan fructotransferase can be produced with high productivity through cost-effective processes
-
medicine
-
the product of the enzymatic reaction di-D-fructose-2,6':2',6 dianhydride stimulates calcium absorption mainly in small intestine of rats and has a practical physiological property in preventing osteoporosis
medicine
-
the product of the enzymatic reaction di-D-fructose-2,6':2',6 dianhydride stimulates calcium absorption mainly in small intestine of rats and has a practical physiological property in preventing osteoporosis
-
synthesis
-
synthesis of di-D-fructose-2,6':2',6 dianhydride
synthesis
-
development of a di-D-fructofranosyl-2,6':2',6-anhydride production system with single culture of Bacillus subtilis directly from sucrose. This system can avoid the purification procedure of levan in which organic solvent is used for precipitation. The levan fructotransferase gene is cloned from Arthrobacter nicotinovorans GS-9 and expressed in levan producing Bacillus subtilis 168. LFTase activity is detected in the culture supernatant of the transformant with maximal activity of 0.062 U/ml after 15.5 h post induction. Then sucrose is added as substrate and incubated. About 78 h after addition of sucrose, 20.5 g/l of di-D-fructofranosyl-2,6':2',6-anhydride is produced from 139.3 g/l of sucrose consumed. The yield of di-D-fructofranosyl-2,6':2',6-anhydride from sucrose is 14.7 wt.%
synthesis
-
enzymatic production of ascorbic acid 2-fructoside, that can be applied to cosmetics, food products, and pharmaceuticals
synthesis
-
synthesis of di-D-fructose-2,6':2',6 dianhydride
-
synthesis
-
development of a di-D-fructofranosyl-2,6':2',6-anhydride production system with single culture of Bacillus subtilis directly from sucrose. This system can avoid the purification procedure of levan in which organic solvent is used for precipitation. The levan fructotransferase gene is cloned from Arthrobacter nicotinovorans GS-9 and expressed in levan producing Bacillus subtilis 168. LFTase activity is detected in the culture supernatant of the transformant with maximal activity of 0.062 U/ml after 15.5 h post induction. Then sucrose is added as substrate and incubated. About 78 h after addition of sucrose, 20.5 g/l of di-D-fructofranosyl-2,6':2',6-anhydride is produced from 139.3 g/l of sucrose consumed. The yield of di-D-fructofranosyl-2,6':2',6-anhydride from sucrose is 14.7 wt.%
-
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Song, K.B.; Bae, K.S.; Lee, Y.B.; Lee, K.Y.; Rhee, S.K.
Characteristics of levan fructotransferase from Arthrobacter ureafaciens K2032 and difructose anhydride IV formation from levan
Enzyme Microb. Technol.
27
212-218
2000
Paenarthrobacter ureafaciens, Paenarthrobacter ureafaciens K2032
brenda
Jang, K.H.; Ryu, E.J.; Park, B.S.; Song, K.B.; Kang, S.A.; Kim, C.H.; Uhm, T.B.; Park, Y.I.; Rhee, S.K.
Levan fructotransferase from Arthrobacter oxydans, J17-21 catalyzes the formation of the di-D-fructose dianhydride IV from levan
J. Agric. Food Chem.
51
2632-2636
2003
Pseudarthrobacter oxydans, Pseudarthrobacter oxydans J17-21
brenda
Saito, K.; Tomita, F.
Difructose anhydrides: Their mass-production and physiological functions
Biosci. Biotechnol. Biochem.
64
1321-1327
2000
Paenarthrobacter nicotinovorans, Paenarthrobacter nicotinovorans GS-9
brenda
Tanaka, K.; Yamaguchi, F.; Kusui, S.
Action of levan fructotransferase of Arthrobacter ureafaciens on levan and phlein
Agric. Biol. Chem.
53
1203-1211
1989
Paenarthrobacter ureafaciens
-
brenda
Tanaka, K.; Akai, T.
Action of levan fructotransferase of Arthrobacter ureafaciens on a mixture of branched levanpentasaccharides
Biosci. Biotechnol. Biochem.
56
814-815
1992
Paenarthrobacter ureafaciens
brenda
Saito, K.; Goto, H.; Yokota, A.; Tomita, F.
Purification of Levan Fructotransferase from Arthvobacter nicotinovoram GS-9 and Production of DFA IV from Levan by the Enzyme.
Biosci. Biotechnol. Biochem.
61
1705-1709
1997
Paenarthrobacter nicotinovorans, Paenarthrobacter nicotinovorans GS-9
brenda
Saito, K.; Yokota, A.; Tomita, F.
Molecular cloning of levan fructotransferase gene from Arthrobacter nicotinovorans GS-9 and its expression in Escherichia coli
Biosci. Biotechnol. Biochem.
61
2076-2079
1997
Paenarthrobacter nicotinovorans, Paenarthrobacter nicotinovorans GS-9
brenda
Yang, S.J.; Park, N.H.; Lee, T.H.; Cha, J.
Expression, purification and characterization of a recombinant levan fructotransferase
Biotechnol. Appl. Biochem.
35
199-203
2002
Microbacterium sp. (Q9EVQ9), Microbacterium sp. AL-210 (Q9EVQ9)
brenda
Lim, S.; Lee, K.Y.; Lee, Y.B.; Song, K.B.
Immobilization of levan fructotransferase for the production of di-fructose anhydride from levan
Biotechnol. Lett.
23
1335-1339
2001
Paenarthrobacter ureafaciens, Paenarthrobacter ureafaciens K2032
-
brenda
Lee, J.; Saraswat, V.; Koh, I.; Song, K.B.; Park, Y.H.; Rhee, S.K.
Secretory production of Arthrobacter levan fructotransferase from recombinant Escherichia coli
FEMS Microbiol. Lett.
195
127-132
2001
Paenarthrobacter ureafaciens, Paenarthrobacter ureafaciens K2032
brenda
Cha, J.; Park, N.H.; Yang, S.J.; Lee, T.H.
Molecular and enzymatic characterization of a levan fructotransferase from Microbacterium sp. AL-210
J. Biotechnol.
91
49-61
2001
Microbacterium sp., Microbacterium sp. AL-210
brenda
Baciu, I.E.; Joerdening, H.J.; Seibel, J.; Buchholz, K.
Investigations of the transfructosylation reaction by fructosyltransferase from B. subtilis NCIMB 11871 for the synthesis of the sucrose analogue galactosyl-fructoside
J. Biotechnol.
116
347-357
2004
Bacillus subtilis, Bacillus subtilis 168
brenda
Kim, C.H.; Jang, E.K.; Kim, S.H.; Jang, K.H.; Kang, S.A.; Song, K.B.; Kwon, O.S.; Rhee, S.K.
Molecular cloning of levan fructotransferase gene from Arthrobacter ureafaciens K2032 and its expression in Escherichia coli for the production of difructose dianhydride IV
Lett. Appl. Microbiol.
40
228-234
2005
Paenarthrobacter ureafaciens, Paenarthrobacter ureafaciens K2032
brenda
Takesue, N.; Sone, T.; Tanaka, M.; Tomita, F.; Asano, K.
Production of di-D-fructofranosyl-2,6:2,6-anhydride (DFA IV) by recombinant Bacillus subtilis carrying heterogenous levan fructotransferase from Arthrobacter nicotinovorans GS-9
Enzyme Microb. Technol.
41
673-676
2007
Paenarthrobacter nicotinovorans, Paenarthrobacter nicotinovorans GS-9
-
brenda
Jang, K.; Jang, E.; Kim, S.; Kim, I.; Kang, S.A.; Koh, I.; Park, Y.; Kim, Y.; Ha, S.; Kim, C.H.
High-level production of low-branched levan from S-4380 for the production of di-beta -D-fructofuranose dianhydride IV
J. Microbiol. Biotechnol.
16
102-108
2006
Pseudomonas chlororaphis subsp. aurantiaca, Pseudomonas chlororaphis subsp. aurantiaca S-4380
-
brenda
Lee, C.Y.; Kim, K.H.; Hur, S.Y.; Heo, J.; Choi, M.H.; Rhee, S.K.; Kim, C.H.
Enzymatic synthesis of ascorbic acid fructoside by transfructosylation using levan fructotransferase
J. Microbiol. Biotechnol.
16
64-67
2006
Paenarthrobacter ureafaciens
-
brenda
Moon, K.O.; Choi, K.H.; Kang, H.Y.; Oh, J.I.; Jang, S.B.; Park, C.S.; Lee, J.H.; Cha, J.
Probing the critical residues for intramolecular fructosyl transfer reaction of a levan fructotransferase
J. Microbiol. Biotechnol.
18
1064-1069
2008
Microbacterium sp.
brenda
Takesue, N.; Sone, T.; Tanaka, M.; Tomita, F.; Asano, K.
Effect of an additionally introduced degQ gene on di-D-fructofuranosyl 2,6:2,6 anhydride (DFA IV) production by recombinant Bacillus subtilis in a single culture production system
J. Biosci. Bioeng.
107
623-629
2009
Paenarthrobacter nicotinovorans
brenda
Kikuchi, H.; Sakurai, H.; Nagura, T.; Aritsuka, T.; Tomita, F.; Yokota, A.
One-pot conversion of levan prepared from Serratia levanicum NN to difructose anhydride IV by Arthrobacter nicotinovorans levan fructotransferase
J. Biosci. Bioeng.
109
240-243
2010
Paenarthrobacter nicotinovorans, Paenarthrobacter nicotinovorans GS9
brenda
Park, J.; Kim, M.I.; Park, Y.D.; Shin, I.; Cha, J.; Kim, C.H.; Rhee, S.
Structural and functional basis for substrate specificity and catalysis of levan fructotransferase
J. Biol. Chem.
287
31233-31241
2012
Paenarthrobacter ureafaciens (Q9KJD0), Paenarthrobacter ureafaciens
brenda