4.2.1.161: bisanhydrobacterioruberin hydratase
This is an abbreviated version!
For detailed information about bisanhydrobacterioruberin hydratase, go to the full flat file.
Reaction
Synonyms
c0505, C50 carotenoid 2'',3''-hydratase, CruF
ECTree
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Engineering
Engineering on EC 4.2.1.161 - bisanhydrobacterioruberin hydratase
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additional information
construction of an enzyme deletion DELTAc0505 mutant strain, the mutant is constructed by homologous recombination. The transcriptions of genes c0507 and c0506 in the DELTAc0505 strain are confirmed by RT-PCR, and the disruption of c0505 does not affect the expression of the upstream genes in the same cluster. In vivo complementation using intact c0505 gene introduced into transformant DELTAc0505(pJc0505), the carotenoid composition of the DELTAc0505(pJc0505) transformant is restored and similar to that of the wild-type
additional information
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construction of an enzyme deletion DELTAc0505 mutant strain, the mutant is constructed by homologous recombination. The transcriptions of genes c0507 and c0506 in the DELTAc0505 strain are confirmed by RT-PCR, and the disruption of c0505 does not affect the expression of the upstream genes in the same cluster. In vivo complementation using intact c0505 gene introduced into transformant DELTAc0505(pJc0505), the carotenoid composition of the DELTAc0505(pJc0505) transformant is restored and similar to that of the wild-type
additional information
-
construction of an enzyme deletion DELTAc0505 mutant strain, the mutant is constructed by homologous recombination. The transcriptions of genes c0507 and c0506 in the DELTAc0505 strain are confirmed by RT-PCR, and the disruption of c0505 does not affect the expression of the upstream genes in the same cluster. In vivo complementation using intact c0505 gene introduced into transformant DELTAc0505(pJc0505), the carotenoid composition of the DELTAc0505(pJc0505) transformant is restored and similar to that of the wild-type
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