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Literature summary for 4.2.1.161 extracted from

  • Yang, Y.; Yatsunami, R.; Ando, A.; Miyoko, N.; Fukui, T.; Takaichi, S.; Nakamura, S.
    Complete biosynthetic pathway of the C50 carotenoid bacterioruberin from lycopene in the extremely halophilic archaeon Haloarcula japonica (2015), J. Bacteriol., 197, 1614-1623 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.161

Cloned(Commentary)

Cloned (Comment) Organism
gene cruF or c0505, functional complementation of enzyme-deficient mutant DELTAc0505 by expression of the intact gene c0505 Haloarcula japonica

Protein Variants

Protein Variants Comment Organism
additional information construction of an enzyme deletion DELTAc0505 mutant strain, the mutant is constructed by homologous recombination. The transcriptions of genes c0507 and c0506 in the DELTAc0505 strain are confirmed by RT-PCR, and the disruption of c0505 does not affect the expression of the upstream genes in the same cluster. In vivo complementation using intact c0505 gene introduced into transformant DELTAc0505(pJc0505), the carotenoid composition of the DELTAc0505(pJc0505) transformant is restored and similar to that of the wild-type Haloarcula japonica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
bacterioruberin Haloarcula japonica overall reaction bisanhydrobacterioruberin + 2 H2O
-
 r
bacterioruberin Haloarcula japonica
-
 monoanhydrobacterioruberin + H2O
-
 r
bacterioruberin Haloarcula japonica JCM 7785 overall reaction bisanhydrobacterioruberin + 2 H2O
-
 r
bacterioruberin Haloarcula japonica JCM 7785
-
 monoanhydrobacterioruberin + H2O
-
 r
monoanhydrobacterioruberin Haloarcula japonica
-
 bisanhydrobacterioruberin + H2O
-
 r
monoanhydrobacterioruberin Haloarcula japonica JCM 7785
-
 bisanhydrobacterioruberin + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Haloarcula japonica A0A0A1GNF2
-
-
Haloarcula japonica JCM 7785 A0A0A1GNF2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bacterioruberin overall reaction Haloarcula japonica bisanhydrobacterioruberin + 2 H2O
-
 r
bacterioruberin overall reaction Haloarcula japonica JCM 7785 bisanhydrobacterioruberin + 2 H2O
-
 r
bacterioruberin
-
 Haloarcula japonica monoanhydrobacterioruberin + H2O
-
 r
bacterioruberin
-
 Haloarcula japonica JCM 7785 monoanhydrobacterioruberin + H2O
-
 r
monoanhydrobacterioruberin
-
 Haloarcula japonica bisanhydrobacterioruberin + H2O
-
 r
monoanhydrobacterioruberin
-
 Haloarcula japonica JCM 7785 bisanhydrobacterioruberin + H2O
-
 r
additional information enzyme C0505 catalyzes the reaction that introduces hydroxyl groups to C3'' and C3''' Haloarcula japonica ?
-
 ?
additional information enzyme C0505 catalyzes the reaction that introduces hydroxyl groups to C3'' and C3''' Haloarcula japonica JCM 7785 ?
-
 ?

Synonyms

Synonyms Comment Organism
c0505
-
 Haloarcula japonica
C50 carotenoid 2'',3''-hydratase
-
 Haloarcula japonica
CruF
-
 Haloarcula japonica

General Information

General Information Comment Organism
evolution C0505, the C50 carotenoid 2'',3''-hydratase from Haloarcula japonica, is a homologue of the CruF-type carotenoid 1,2-hydratase Haloarcula japonica
metabolism the gene cluster, including genes c0507, c0506, and c0505, is involved in the synthesis of bacterioruberin. Genes c0507, c0506, and c0505 encode a carotenoid 3,4-desaturase (CrtD), a bifunctional lycopene elongase and 1,2-hydratase (LyeJ), and a C50 carotenoid 2'',3''-hydratase (CruF), respectively. The three carotenoid biosynthetic enzymes catalyze the reactions that convert lycopene to bacterioruberin in Haloarcula japonica. Biosynthetic pathway of C50 carotenoids, overview Haloarcula japonica