4.2.1.135: UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining)

This is an abbreviated version!
For detailed information about UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining), go to the full flat file.

Reaction

Synonyms

Bc3750, BC_3750, Cj1120c, Pdeg, PglF, UDP-Glc-NAc 4,6-dehydratase, UDP-N-acetylglucosamine C4,6-dehydratase

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.135 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining)

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Results	in table
1	Activating Compound
1082	AA Sequence
2	Application
5	Cloned(Commentary)
3	Cofactor
8	General Information
5	Inhibitors
1	KM Value [mM]
2	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
18	Organism
8	Pathway
4	pH Optimum
4	Protein Variants
3	Purification (Commentary)
1	Reaction
9	Reference
11	Substrates and Products (Substrate)
2	Subunits
25	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Stability [°C]
1	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 4.2.1.135 - UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining)

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

UDP-N-acetyl-alpha-D-glucosamine

UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O

4 entries

UDP-N-acetylglucosamine

?

Campylobacter jejuni

-

-

714453

-

-

?

UDP-N-acetylglucosamine

UDP-2-acetamido-2,6-dideoxy-alpha-D-4-ketohexulose + H2O

Campylobacter jejuni

-

-

692984

-

-

?

additional information

?

-

3 entries

UDP-N-acetyl-alpha-D-glucosamine

UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O

Bacillus cereus

Q81A42

-

748586, 749074

-

-

?

UDP-N-acetyl-alpha-D-glucosamine

UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O

Bacillus cereus ATCC 14579

-

-

748586

-

-

?

UDP-N-acetyl-alpha-D-glucosamine

UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O

Bacillus cereus ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711

Q81A42

-

748586, 749074

-

-

?

UDP-N-acetyl-alpha-D-glucosamine

UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O

Campylobacter jejuni

-

PglF performs a cofactor-dependent hydride transfer from the C4 position of UDP-GlcNAc to C6, in conjunction with the elimination of water across the glucosyl C5 and C6 bond

714160

-

-

?

additional information

?

-

Bacillus cereus

Q81A42

product identification by mass spectrometry and one- and two-dimensional NMR analysis, substrate specificity analysis, overview. None of the UDP-sugars tested including UDP-galactose, UDPGalNAc, UDP-GlcNAc, and UDP-glucose are substrates for recombinant Pdeg

749074

-

-

?

additional information

?

-

Bacillus cereus ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711

Q81A42

product identification by mass spectrometry and one- and two-dimensional NMR analysis, substrate specificity analysis, overview. None of the UDP-sugars tested including UDP-galactose, UDPGalNAc, UDP-GlcNAc, and UDP-glucose are substrates for recombinant Pdeg

749074

-

-

?

additional information

?

-

Campylobacter jejuni

-

PglF catalyzes the formation of a UDP-4-oxo sugar with the appropriate stereochemistry at position 5'' from UDP-GlcNAc. Sugar capsule capsular polysaccharide A (CPSA), which coats the surface of the mammalian symbiont Bacteroides fragilis, is a key mediator of mammalian immune system development, the enzyme, coupled to a Bacteroides fragilis-encoded aminotransferase (WcfR), is used in sythetic construction system for CPSA for synthesis of the rare stereoconfiguration sugar acetamido-4-amino-6-deoxygalactopyranose in a two-step coupled reaction with WcfR, overview

729230

-

-

?