4.2.1.126: N-acetylmuramic acid 6-phosphate etherase

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For detailed information about N-acetylmuramic acid 6-phosphate etherase, go to the full flat file.

Reaction

Synonyms

etherase, MurNAc 6-phosphate etherase, MurNAc 6-phosphate etherase lyase, MurNAc 6-phosphate hydrolase, MurNAc etherase, MurNAc-6-P etherase, MurNAc-6-phosphate etherase, MurQ, MurQ etherase, MurQ hydrolase, N-acetylmuramic acid 6-phosphate hydrolase, N-acetylmuramic acid 6-phosphate lyase, N-acetylmuramic acid-phosphate etherase, Tanf_08385, TfMurQ, YbbI

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.126 N-acetylmuramic acid 6-phosphate etherase

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Results	in table
14922	AA Sequence
4	Cloned(Commentary)
1	Crystallization (Commentary)
14	General Information
3	Inhibitors
3	kcat/KM [mM/s]
2	Ki Value [mM]
3	KM Value [mM]
6	Localization
4	Natural Substrates/ Products (Substrates)
131	Organism
3	Pathway
11	PDB ID
2	pH Optimum
3	Protein Variants
4	Purification (Commentary)
3	Reaction
2	Reaction Type
15	Reference
3	Storage Stability
24	Substrates and Products (Substrate)
1	Subunits
40	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
4	Turnover Number [1/s]

Cloned

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Cloned on EC 4.2.1.126 - N-acetylmuramic acid 6-phosphate etherase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Escherichia coli BL21(DE3) cells

Escherichia coli

-

714166

gene murQ, DNA and amino acid sequence determination and analysis, genetic organization of the MurNAc utilization locus, the three genes, Tanf_08375, Tanf_08380, and Tanf_08385 (murQ), encoding a MurNAc transporter, a putative sugar kinase, and a MurQ etherase, respectively, are transcriptionally linked. Complementation with the Tanf_08375 and Tanf_08380 genes together in trans, but not individually, rescues the inability of an Escherichia coli mutant deficient in the phosphotransferase (PTS) system-dependent MurNAc transporter MurP as well as that of a double mutant deficient in MurP and components of the PTS system to grow on MurNAc. In addition, complementation with this two-gene construct in Escherichia coli causes depletion of MurNAc in the medium. Recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)

Tannerella forsythia

G8UQI0

748107

gene MurQ-HI, functional expression of N-terminally His6-tagged enzyme in Escherichia coli

Haemophilus influenzae

P44862

729247

MurQ-His fusion protein is expressed in Escherichia coli BL21 cells

Escherichia coli

-

715477