4.2.1.126: N-acetylmuramic acid 6-phosphate etherase

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For detailed information about N-acetylmuramic acid 6-phosphate etherase, go to the full flat file.

Reaction

Synonyms

etherase, MurNAc 6-phosphate etherase, MurNAc 6-phosphate etherase lyase, MurNAc 6-phosphate hydrolase, MurNAc etherase, MurNAc-6-P etherase, MurNAc-6-phosphate etherase, MurQ, MurQ etherase, MurQ hydrolase, N-acetylmuramic acid 6-phosphate hydrolase, N-acetylmuramic acid 6-phosphate lyase, N-acetylmuramic acid-phosphate etherase, Tanf_08385, TfMurQ, YbbI

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.126 N-acetylmuramic acid 6-phosphate etherase

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Results	in table
14922	AA Sequence
4	Cloned(Commentary)
1	Crystallization (Commentary)
14	General Information
3	Inhibitors
3	kcat/KM [mM/s]
2	Ki Value [mM]
3	KM Value [mM]
6	Localization
4	Natural Substrates/ Products (Substrates)
131	Organism
3	Pathway
11	PDB ID
2	pH Optimum
3	Protein Variants
4	Purification (Commentary)
3	Reaction
2	Reaction Type
15	Reference
3	Storage Stability
24	Substrates and Products (Substrate)
1	Subunits
40	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
4	Turnover Number [1/s]

Crystallization

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Crystallization on EC 4.2.1.126 - N-acetylmuramic acid 6-phosphate etherase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant enzyme as apoenzyme and in complex with inhibitor (2R)-2-[[(2R,3R,4R)-5-(acetylamino)-2,3,7-trihydroxy-1-(phosphonooxy)heptan-4-yl]oxy]propanoic acid, sitting drop vapor diffusion method, mixing of 8 mg/mL protein in 200 mM NaCl, 50 mM HEPES, and 5% glycerol with 0.2 M NaCl, 0.1 M Bis-Tris-HCl, pH 5.5, and 25% w/v PEG 3350 or with 0.17 M ammonium sulfate, 25.5% w/v PEG 4000, and 15% v/v glycerol, the former gives better results, soaking of crystals in solution containing the inhibitor, 25°C, X-ray diffraction structure determinnation and analysis at 2.6 A and 2.4 A resolution, respectively, molecular replacement using PDB entry 1NRI as the template

Haemophilus influenzae

P44862

729247