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4.2.1.118: 3-dehydroshikimate dehydratase

This is an abbreviated version!
For detailed information about 3-dehydroshikimate dehydratase, go to the full flat file.

Word Map on EC 4.2.1.118

Reaction

3-dehydro-shikimate
=
3,4-dihydroxybenzoate
 +
H2O

Synonyms

(-)-3-dehydroshikimate dehydratase, 3-dehydroshikimate dehydratase, AroZ, AsbF, BT246_21860, dehydroshikimate dehydratase, DHSase, DSD, mDSD, membrane-bound 3-dehydroshikimate dehydratase, Qa-1, QsuB, Qui1, quiC1, quiC_1, QutC

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.118 3-dehydroshikimate dehydratase

Engineering

Engineering on EC 4.2.1.118 - 3-dehydroshikimate dehydratase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
T61N/H135Y/H257P
random mutagenesis, triple AsbF mutant Mut1, half-life of Mut1 at 37°C is over 10fold higher compared to wild-type AsbF. The second-order rate constants for both wild-type AsbF and Mut1 are approximately equal, thus demonstrating protein thermostability does not come at the expense of enzyme thermophilicity
E356A
site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme
H168A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
R207A
site-directed mutagenesis, the mutant shows moderately reduced activity compared to the wild-type enzyme
R210A
site-directed mutagenesis, inactive mutant
S206A
site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme
additional information