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Catalyses an early step in the biosynthesis of petrobactin, a siderophore produced by many bacteria, including the human pathogen Bacillus anthracis. Requires divalent ions, with a preference for Mn2+.
constitutve overexpression leads to impaired growth in minimal medium which can be alleviated by the addition of aromatic amino acids to the medium. Overexpression of the qutC gene in mutant strains lacking protocatechuic acid oxygenase leads to the build up of protocatechuic acid in the medium. The metabolites 3-dehydroquinate and dehydroshikimate leak from the pentafunctional AROM protein at a rate comparable with the extent of flux catalysed by the AROM protein. The AROM protein has a low level channelling function probably as a result of the close juxtaposition of five active sites and this channelling function is only physiologically significant under non-optimal conditions of nutrient supply and oxygenation, when the organism is in situ in its natural environment
native mDSD from strain IFO 3244 membrane fraction to homogeneity by solubilization with detergents n-dodecyl-beta-D-maltoside and n-octyl-beta-D-glucoside, anion exchange and hydroxyapatite chromatography. The cytosolic isozyme sDSD is purified from the cytosolic fraction after centrifugation at 62000 x g followed by anion echange chromatograohy, ammonium sulfate precipitation, and dialysis, the enzyme activity is lost during dialysis
production of vanillin by engineered pathway in Schizosaccharomyces pombe or Saccharomyces cerevisiae. Pathway involves incorporation of 3-dehydroshikimate dehydratase, an aromatic carboxylic acid reductase from a bacterium of the Nocardia genus, and an O-ethyltransferase from Homo sapiens. In Saccharomyces cerevisiae, the aromatic carboxylic acid reductase enzyme requires activation by phosphopantetheinylation, achieved by coexpression of a Corynebacterium glutamicum phosphopantetheinyl transferase. Prevention of reduction of vanillin to vanillyl alcohol is achieved by knockout of the host alcohol dehydrogenase ADH6. In Schizosaccharomyces pombe, the biosynthesis is further improved by introduction of an Arabidopsis thaliana family 1 UDPglycosyltransferase, converting vanillin into vanillin beta-D-glucoside, which is not toxic to the yeast cells and thus may be accumulated in larger amounts
introduction of both 3-dehydroshikimate dehydratase and protocatechuic acid decarboxylase into an Escherichia coli construct synthesizing elevated levles of 3-dehydroshikimic acid leads to production of up to 18.5 mM catechol from 56 mM D-glucose on 1 l-scale