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Literature summary for 4.2.1.118 extracted from

  • Hansen, E.H.; Moeller, B.L.; Kock, G.R.; Bunner, C.M.; Kristensen, C.; Jensen, O.R.; Okkels, F.T.; Olsen, C.E.; Motawia, M.S.; Hansen, J.
    De novo biosynthesis of vanillin in fission yeast (Schizosaccharomyces pombe) and baker's yeast (Saccharomyces cerevisiae) (2009), Appl. Environ. Microbiol., 75, 2765-2774.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis production of vanillin by engineered pathway in Schizosaccharomyces pombe or Saccharomyces cerevisiae. Pathway involves incorporation of 3-dehydroshikimate dehydratase, an aromatic carboxylic acid reductase from a bacterium of the Nocardia genus, and an O-ethyltransferase from Homo sapiens. In Saccharomyces cerevisiae, the aromatic carboxylic acid reductase enzyme requires activation by phosphopantetheinylation, achieved by coexpression of a Corynebacterium glutamicum phosphopantetheinyl transferase. Prevention of reduction of vanillin to vanillyl alcohol is achieved by knockout of the host alcohol dehydrogenase ADH6. In Schizosaccharomyces pombe, the biosynthesis is further improved by introduction of an Arabidopsis thaliana family 1 UDPglycosyltransferase, converting vanillin into vanillin beta-D-glucoside, which is not toxic to the yeast cells and thus may be accumulated in larger amounts Podospora pauciseta

Organism

Organism UniProt Comment Textmining
Podospora pauciseta
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