4.1.3.39: 4-hydroxy-2-oxovalerate aldolase
This is an abbreviated version!
For detailed information about 4-hydroxy-2-oxovalerate aldolase, go to the full flat file.
Word Map on EC 4.1.3.39
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4.1.3.39
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hydratase
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meta-cleavage
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catechols
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4-oxalocrotonate
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2-hydroxymuconic
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2,3-dioxygenase
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2-hydroxypenta-2,4-dienoate
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putida
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biphenyls
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semialdehyde
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dioxygenase
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extradiol
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enol
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tautomerase
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polychlorinated
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ethylbenzene
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meta-pathway
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isofunctional
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stearothermophilus
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xylf
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propionaldehyde
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aldolases
- 4.1.3.39
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hydratase
-
meta-cleavage
- catechols
- 4-oxalocrotonate
-
2-hydroxymuconic
-
2,3-dioxygenase
- 2-hydroxypenta-2,4-dienoate
- putida
- biphenyls
- semialdehyde
- dioxygenase
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extradiol
-
enol
-
tautomerase
-
polychlorinated
- ethylbenzene
-
meta-pathway
-
isofunctional
- stearothermophilus
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xylf
- propionaldehyde
- aldolases
Reaction
Synonyms
4-hydroxy-2-ketovalerate aldolase, 4-hydroxy-2-ketovalerate aldolase-aldehyde dehydrogenase (acylation), BphI, class II pyruvate aldolase BphI, DmpFG, HOA, HsaF, MhpE, pyruvate aldolase
ECTree
4.1.3.39 4-hydroxy-2-oxovalerate aldolaseAdvanced search results
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results (KCat KM Value
KCat KM Value on EC 4.1.3.39 - 4-hydroxy-2-oxovalerate aldolase
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kcat/KM VALUE [1/mMs-1] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
79.1
(S)-4-hydroxy-2-oxohexanoate
in 100 mM HEPES (pH 8.0), 0.4 mM NADH, 1 mM MgCl2, at 25°C
93.1
(S)-4-hydroxy-2-oxopentanoate
in 100 mM HEPES (pH 8.0), 0.4 mM NADH, 1 mM MgCl2, at 25°C
0.0005
Isobutyraldehyde
at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate
10.4
(4R)-4-hydroxy-2-oxopentanoate
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Y290F mutant protein, pH 8.0, 25°C
14.1
(4R)-4-hydroxy-2-oxopentanoate
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Y290S mutant protein, pH 8.0, 25°C
0.012
(4S)-4-hydroxy-2-oxopentanoate
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R16K mutant protein, pH 8.0, 25°C
0.34
(4S)-4-hydroxy-2-oxopentanoate
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H20S mutant protein, pH 8.0, 25°C
0.45
(4S)-4-hydroxy-2-oxopentanoate
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H20A mutant protein, pH 8.0, 25°C
12.6
(4S)-4-hydroxy-2-oxopentanoate
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Y290F mutant protein, pH 8.0, 25°C
13.4
(4S)-4-hydroxy-2-oxopentanoate
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Y290S mutant protein, pH 8.0, 25°C
44.7
(4S)-4-hydroxy-2-oxopentanoate
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wild type protein, pH 8.0, 25°C
0.0134
acetaldehyde
at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate
0.00219
Butyraldehyde
at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate
0.0037
glycolaldehyde
at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate
0.00047
pentaldehyde
at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate
0.0132
propionaldehyde
at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate
0.0159
pyruvate
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aldol addition reaction with propionaldehyde, L87A mutant protein, pH 8.0, 25°C
0.0587
pyruvate
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aldol addition reaction with propionaldehyde, L89A mutant protein, pH 8.0, 25°C
0.0915
pyruvate
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aldol addition reaction with propionaldehyde, wild type protein, pH 8.0, 25°C
