4.1.3.39: 4-hydroxy-2-oxovalerate aldolase

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For detailed information about 4-hydroxy-2-oxovalerate aldolase, go to the full flat file.

Word Map on EC 4.1.3.39

4.1.3.39

hydratase

meta-cleavage

catechols

4-oxalocrotonate

2-hydroxymuconic

2,3-dioxygenase

2-hydroxypenta-2,4-dienoate

putida

biphenyls

semialdehyde

dioxygenase

extradiol

enol

tautomerase

polychlorinated

ethylbenzene

meta-pathway

isofunctional

stearothermophilus

xylf

propionaldehyde

aldolases

Reaction

Synonyms

4-hydroxy-2-ketovalerate aldolase, 4-hydroxy-2-ketovalerate aldolase-aldehyde dehydrogenase (acylation), BphI, class II pyruvate aldolase BphI, DmpFG, HOA, HsaF, MhpE, pyruvate aldolase

ECTree

4 Lyases

4.1 Carbon-carbon lyases

4.1.3 Oxo-acid-lyases

4.1.3.39 4-hydroxy-2-oxovalerate aldolase

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Results	in table
4	Activating Compound
7344	AA Sequence
1	CAS Registry Number
7	Cloned(Commentary)
1	Cofactor
3	Crystallization (Commentary)
3	General Information
16	Inhibitors
50	kcat/KM [mM/s]
15	Ki Value [mM]
36	KM Value [mM]
10	Metals/Ions
7	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
38	Organism
13	Pathway
5	PDB ID
3	pH Optimum
2	pH Range
9	Protein Variants
5	Purification (Commentary)
7	Reaction
17	Reference
2	Specific Activity [micromol/min/mg]
2	Storage Stability
69	Substrates and Products (Substrate)
14	Subunits
21	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
36	Turnover Number [1/s]

Crystallization

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Crystallization on EC 4.1.3.39 - 4-hydroxy-2-oxovalerate aldolase

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in complex with HsaG, sitting drop vapor diffusion method, using 18% (w/v) PEG 4000, 0.2 M MgCl2, 0.1 M Tris pH 8.5, 10% glycerol, and 10 mM sodium oxalate

Mycobacterium tuberculosis

P9WMK5

724380

purified enzyme complex of 4-hydroxy-2-ketovalerate aldolase and aldehyde dehydrogenase (acylating), protein solution contains 9 mg/ml protein in 50 mM Tris-HCl, pH 7.4, 1 mM DTT, and 2 mM NAD+, mixing of equal volumes of 0.001 ml of protein and reservoir solutions, the latter contains 15% PEG 8000, 100 mM ammonium sulfate, and 100 mM PIPES, pH 7.5, 3 days, soaking of crystals in 5 mM samarium acetate and 0.25 mM PCMBS, X-ray diffraction structure determination and analysis at 2.1 A resolution

Pseudomonas sp.

390178

purified selenomethionine-labeled enzyme complex of 4-hydroxy-2-ketovalerate aldolase and aldehyde dehydrogenase (acylating), protein solution contains 9 mg/ml protein in 50 mM Tris-HCl, pH 7.4, 1 mM DTT, and 2 mM NAD+, mixing of equal volumes of 0.001 ml of protein and reservoir solutions, the latter contains 18% PEG 8000, 100 mM ammonium sulfate, and 100 mM PIPES, pH 7.5, 3 days, crystals are used for microseeding, cryoprotection by soaking in mother liquor with 20% v/v 2-methyl-2,4-pentanediol, X-ray diffraction structure determination and analysis at 1.7 A resolution

Pseudomonas sp.

657161