4.1.3.38: aminodeoxychorismate lyase

This is an abbreviated version!
For detailed information about aminodeoxychorismate lyase, go to the full flat file.

Reaction

Synonyms

4-amino-4-deoxychorismate lyase, Abz2, ADC lyase, ADCL, ADCS, aminodeoxychorismate lyase, aminodeoxychorismate synthase, enzyme X, PabC, PabC-1, PabC-2, TTHA0621 protein

ECTree

4 Lyases

4.1 Carbon-carbon lyases

4.1.3 Oxo-acid-lyases

4.1.3.38 aminodeoxychorismate lyase

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Results	in table
5093	AA Sequence
1	Application
1	CAS Registry Number
10	Cloned(Commentary)
11	Cofactor
4	Crystallization (Commentary)
7	General Information
2	Inhibitors
2	Localization
6	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
23	Organism
5	Pathway
5	PDB ID
1	pH Optimum
8	Protein Variants
9	Purification (Commentary)
4	Reaction
1	Reaction Type
21	Reference
24	Substrates and Products (Substrate)
5	Subunits
37	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]

Purification

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Purification on EC 4.1.3.38 - aminodeoxychorismate lyase

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DEAE-Sephacel, phenyl-Sepharose column, Superose 6 gel filtration column, Mono Q, Superose 12 column, yield 400-800fold

Escherichia coli

644227

fractionation with ammonium sulfate pH 7.5, DEAE-Sephacel column, butyl-Sepharose 4B column, Gigapite column

Escherichia coli

644232

fractionation with ammonium sulfate, DEAE-Toyopearl column, butyl-Toyopearl column and Mono Q column

Escherichia coli

644231

nickel affinity column chromatography

Streptomyces sp.

B8Y8J0

705561

reactive yellow 3-agarose column, Mono Q HR 5/5 FPLC column, Superose 12HR 10/30 FPLC column, Mono Q HR 5/20 FPLC column, Aquapore RP-300 C8 HPLC column: 4100fold to near homogeneity

Escherichia coli

644229

recombinant His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli strain BL21(DE3) and B834(DE3), respectively, by nickel affinity chromatography

Saccharomyces cerevisiae

Q03266

727950

recombinant His6-tagged enzyme from Escherichia coli by metal affinity chromatography

Plasmodium falciparum

Q8IKP7

726913

recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) GOLD by nickel affinity chromatography, cleavage of the tag by TEV protease, and gel filtration

Pseudomonas aeruginosa

Q9HZN6

728557

Resource ISO column chromatography, ammonium sulfate precipitation, Resource Q column chromatography, and Superdex 75 gel filtration

Thermus thermophilus

Q5SKM2

701506