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4.1.3.38: aminodeoxychorismate lyase

This is an abbreviated version!
For detailed information about aminodeoxychorismate lyase, go to the full flat file.

Reaction

4-amino-4-deoxychorismate
=
4-Aminobenzoate
+
pyruvate

Synonyms

4-amino-4-deoxychorismate lyase, Abz2, ADC lyase, ADCL, ADCS, aminodeoxychorismate lyase, aminodeoxychorismate synthase, enzyme X, PabC, PabC-1, PabC-2, TTHA0621 protein

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.3 Oxo-acid-lyases
                4.1.3.38 aminodeoxychorismate lyase

Cloned

Cloned on EC 4.1.3.38 - aminodeoxychorismate lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNAs are shown to encode functional enzymes by complementation of an Escherichia coli pabC mutant, and by demonstrating that the partially purified recombinant proteins convert 4-amino-4-deoxychorismate to 4-aminobenzoate
-
cDNAs are shown to encode functional enzymes by complementation of an Escherichia coli pabC mutant, and by demonstrating that the partially purified recombinant proteins convert 4-amino-4-deoxychorismate to 4-aminobenzoate. The full-length Arabidopsis ADC lyase polypeptide is translocated into isolated pea chloroplasts and, when fused to green fluorescent protein, directed the passenger protein to Arabidopsis chloroplasts in transient expression experiments
-
expressed in Escherichia coli B834 (DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli JM109
-
expression in Escherichia coli MC1000 cells
gene pabC, expression of His6-tagged enzyme with TEV protease cleavage site in Escherichia coli strain BL21(DE3) GOLD
gene PF14_0557, functional overexpression as His6-tagged protein in Escherichia coli cells
recombinant expression of His-tagged wild-type and selenomethionine-labeled enzymes in Escherichia coli strain BL21(DE3) and B834(DE3), respectively