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0.39 - 11
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
0.35 - 10.6
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
0.2 - 11
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
3.5 - 14.5
D-3-deoxy-manno-2-octulosonic acid
2.4 - 14.1
L-3-deoxy-manno-2-octulosonic acid
12.5
N-acetyl-4-O-acetylneuraminic acid
-
-
4.5
N-Acetyl-7-O-acetylneuraminic acid
-
-
2
N-Acetyl-8-O-acetylneuraminic acid
-
-
6.9 - 3200
N-acetyl-D-mannosamine
2.1 - 38
N-acetyl-D-neuraminic acid
3 - 369.4
N-acetyl-L-neuraminic acid
2.8
N-acetyl-neuraminic acid
-
in 0.1 M phosphate buffer, pH 7
0.013 - 87.7
N-acetylneuraminate
0.4 - 1100
N-acetylneuraminic acid
1.75
N-Formylneuraminic acid
-
-
2.2 - 4.6
N-glycolylneuraminic acid
2.7
N-Monochloracetylneuraminic acid
-
-
10
N-Monofluoracetylneuraminic acid
-
-
1.93
N-Succinylneuraminic acid
-
-
additional information
additional information
-
with substrate N-acetylneuraminic acid increasing Km with increasing pH
-
0.39
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
-
mutant enzyme E192N
11
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
-
wild-type enzyme
0.35
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, mutant enzyme E192V
0.39
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, mutant enzyme E192N
0.5
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, mutant enzyme E192S
2.54
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, mutant enzyme S208G
5.7
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, mutant enzyme D191P
10.6
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, wild-type enzyme
0.2
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192M mutant protein, pH 7.5, 25°C
0.2
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192P mutant protein, pH 7.5, 25°C
0.3
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192F mutant protein, pH 7.5, 25°C
0.4
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192N mutant protein, pH 7.7.5, 25°C
0.4
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192V mutant protein, pH 7.5, 25°C
0.5
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192H mutant protein, pH 7.5, 25°C
0.6
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192S mutant protein, pH 7.5, 25°C
0.7
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192Q mutant protein, pH 7.5, 25°C
1.1
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192D mutant protein, pH 7.5, 25°C
11
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
wild type (E192), pH 7.5, 25°C
3.5
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
6.3
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
11.4
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
12.3
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L
13.8
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, wild-type enzyme
14.5
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
2.4
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
2.7
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
2.8
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
7.5
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, wild-type enzyme
9.2
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
14.1
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L
6.9
N-acetyl-D-mannosamine
at pH 7.0 and 37°C
7.7
N-acetyl-D-mannosamine
-
-
53.3
N-acetyl-D-mannosamine
at pH 7.5 and 37°C
92.1
N-acetyl-D-mannosamine
at pH 8.5 and 37°C
131.7
N-acetyl-D-mannosamine
-
recombinant enzyme, pH and temperature not specified in the publication
149
N-acetyl-D-mannosamine
recombinant His-taggged enzyme, pH 7.0, 37°C
160
N-acetyl-D-mannosamine
-
pH 7.0, 37°C
160
N-acetyl-D-mannosamine
-
soluble enzyme, pH not specified in the publication, temperature not specified in the publication
180
N-acetyl-D-mannosamine
-
220
N-acetyl-D-mannosamine
-
245
N-acetyl-D-mannosamine
-
cross-linked enzyme aggregates, pH 7.5, 37°C
272
N-acetyl-D-mannosamine
-
wild type enzyme, at pH 7.0 and 37°C
333
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
915
N-acetyl-D-mannosamine
mutant enzyme P193Y, at pH 7.0 and 37°C
3200
N-acetyl-D-mannosamine
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
2.1
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L
2.6
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, wild-type enzyme
3.9
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
4.4
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, wild-type enzyme
10.1
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
10.7
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme S208G
12.1
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
15.3
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
17
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme E192S
34
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme E192V
38
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme E192N
3 - 3.5
N-acetyl-L-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
4.4
N-acetyl-L-neuraminic acid
-
pH 7.5, 25°C, wild-type enzyme
36.2
N-acetyl-L-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
127
N-acetyl-L-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
369.4
N-acetyl-L-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
0.013
N-acetylneuraminate
purified recombinant mutant K165C, pH 7.4, 30°C
0.015
N-acetylneuraminate
purified recombinant mutant K165-gamma-thialysine, pH 6.8, 30°C
0.023
N-acetylneuraminate
purified recombinant mutant K165-gamma-thialysine, pH 7.4, 30°C
0.036
N-acetylneuraminate
purified recombinant wild-type enzyme, pH 7.4, 30°C
0.04
N-acetylneuraminate
purified recombinant wild-type enzyme, pH 6.8, 30°C
0.27
N-acetylneuraminate
mutant enzyme P193Y, at pH 7.0 and 37°C
0.32
N-acetylneuraminate
-
wild type enzyme, at pH 7.0 and 37°C
1.1
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
1.2
N-acetylneuraminate
mutant enzyme G211S, at pH 7.0 and 37°C
1.8
N-acetylneuraminate
at pH 7.0 and 37°C
2
N-acetylneuraminate
recombinant His-taggged enzyme, pH 7.0, 37°C
2
N-acetylneuraminate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
2.2
N-acetylneuraminate
-
wild-type enzyme, pH 7.4, 30°C
2.5
N-acetylneuraminate
-
2.51
N-acetylneuraminate
mutant enzyme L171N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
3 - 3.5
N-acetylneuraminate
at pH 7.5 and 37°C
3.2
N-acetylneuraminate
mutant enzyme L199N/I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
3.2
N-acetylneuraminate
at 30°C, pH not specified in the publication
3.4
N-acetylneuraminate
mutant enzyme I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
3.42
N-acetylneuraminate
mutant enzyme L171R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
3.7
N-acetylneuraminate
-
mutant Y137F enzyme, pH 7.4, 30°C
3.93
N-acetylneuraminate
mutant enzyme I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
4
N-acetylneuraminate
mutant enzyme L199N/I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
4
N-acetylneuraminate
-
recombinant enzyme, pH and temperature not specified in the publication
4.1
N-acetylneuraminate
wild type enzyme, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
4.3
N-acetylneuraminate
mutant enzyme L199N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
4.7
N-acetylneuraminate
mutant enzyme L199N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
4.9
N-acetylneuraminate
-
5
N-acetylneuraminate
mutant enzyme I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
6.1
N-acetylneuraminate
mutant enzyme L199R/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
87.7
N-acetylneuraminate
at pH 8.5 and 37°C
0.4
N-acetylneuraminic acid
-
pH 7.2, Y133F mutant
1
N-acetylneuraminic acid
-
pH 7.2, Y133H mutant
1
N-acetylneuraminic acid
-
pH 7.4, 75°C
1.4
N-acetylneuraminic acid
-
pH 7.4, 37°C
1.5 - 3.7
N-acetylneuraminic acid
-
-
1.7
N-acetylneuraminic acid
-
pH 7.4, 37°C
1.75
N-acetylneuraminic acid
-
-
1.8
N-acetylneuraminic acid
-
-
1.8
N-acetylneuraminic acid
-
pH 7.0, 37°C
1.9 - 3.9
N-acetylneuraminic acid
-
-
2
N-acetylneuraminic acid
-
pH 7.2, 50 mM phosphate buffer, wild-type
2.4
N-acetylneuraminic acid
-
-
2.4
N-acetylneuraminic acid
-
pH 7.2, Y133C mutant
2.8
N-acetylneuraminic acid
-
-
2.9
N-acetylneuraminic acid
-
pH 7.2, Y133W mutant
3.2
N-acetylneuraminic acid
-
pH 7.2, 250 mM phosphate buffer, wild-type
3.3
N-acetylneuraminic acid
-
-
3.3
N-acetylneuraminic acid
-
-
3.5
N-acetylneuraminic acid
-
pH 7.2, K161R mutant
3.5
N-acetylneuraminic acid
-
E192Q mutant protein, pH 7.5, 25°C
3.6
N-acetylneuraminic acid
-
-
3.7
N-acetylneuraminic acid
-
-
3.9
N-acetylneuraminic acid
-
-
4.4
N-acetylneuraminic acid
-
wild type (E192), pH 7.5, 25°C
4.6
N-acetylneuraminic acid
-
E192D mutant protein, pH 7.5, 25°C
4.8
N-acetylneuraminic acid
-
-
5
N-acetylneuraminic acid
-
E192M mutant protein, pH 7.5, 25°C
5.9
N-acetylneuraminic acid
-
E192F mutant protein, pH 7.5, 25°C
6
N-acetylneuraminic acid
-
E192P mutant protein, pH 7.5, 25°C
6
N-acetylneuraminic acid
-
E192S mutant protein, pH 7.5, 25°C
6.2
N-acetylneuraminic acid
-
E192H mutant protein, pH 7.5, 25°C
17
N-acetylneuraminic acid
-
pH 7.2, E188Q mutant
29
N-acetylneuraminic acid
-
pH 7.2, D187E mutant
34
N-acetylneuraminic acid
-
E192V mutant protein, pH 7.5, 25°C
38
N-acetylneuraminic acid
-
E192N mutant protein, pH 7.7.5, 25°C
44
N-acetylneuraminic acid
-
pH 7.2, E188D mutant
1100
N-acetylneuraminic acid
-
pH 7.2, D187N mutant
2.2
N-glycolylneuraminic acid
-
-
3.3
N-glycolylneuraminic acid
-
-
4.3
N-glycolylneuraminic acid
-
-
4.6
N-glycolylneuraminic acid
-
-
0.5
pyruvate
mutant enzyme P193Y, at pH 7.0 and 37°C
1 - 4
pyruvate
recombinant His-taggged enzyme, pH 7.0, 37°C
1.1
pyruvate
wild type enzyme, at pH 7.0 and 37°C
4.3
pyruvate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
5.9
pyruvate
at pH 7.0 and 37°C
9
pyruvate
-
cross-linked enzyme aggregates, pH 7.5, 37°C
12.5
pyruvate
-
wild type enzyme, at pH 7.0 and 37°C
14.7
pyruvate
at pH 7.5 and 37°C
19.9
pyruvate
-
pH 7.0, 37°C
19.9
pyruvate
-
soluble enzyme, pH not specified in the publication, temperature not specified in the publication
35.1
pyruvate
-
recombinant enzyme, pH and temperature not specified in the publication
72.4
pyruvate
at pH 8.5 and 37°C
4.4
sialic acid
-
wild-type enzyme
38
sialic acid
-
mutant enzyme E192N