Application | Comment | Organism |
---|---|---|
biotechnology | cross-linked enzyme aggregates | Lactiplantibacillus plantarum |
Cloned (Comment) | Organism |
---|---|
His-tagged version expressed in Escherichia coli BL21(DE3)pLys | Lactiplantibacillus plantarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
pyruvate | cross-linked enzyme aggregates, pH 7.5, 37°C | Lactiplantibacillus plantarum | |
19.9 | - |
pyruvate | soluble enzyme, pH not specified in the publication, temperature not specified in the publication | Lactiplantibacillus plantarum | |
160 | - |
N-acetyl-D-mannosamine | soluble enzyme, pH not specified in the publication, temperature not specified in the publication | Lactiplantibacillus plantarum | |
245 | - |
N-acetyl-D-mannosamine | cross-linked enzyme aggregates, pH 7.5, 37°C | Lactiplantibacillus plantarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | - |
- |
- |
Lactiplantibacillus plantarum WFCS1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lactiplantibacillus plantarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-neuraminic acid | - |
Lactiplantibacillus plantarum | pyruvate + N-acetyl-D-mannosamine | - |
r | |
N-acetyl-D-neuraminic acid | - |
Lactiplantibacillus plantarum WFCS1 | pyruvate + N-acetyl-D-mannosamine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
N-acetyl-d-neuraminic acid aldolase | - |
Lactiplantibacillus plantarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
cross-linked enzyme aggregates, stable at pH 9.0 for 6 hours as the soluble enzyme | Lactiplantibacillus plantarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
soluble enzyme | Lactiplantibacillus plantarum |
7.5 | - |
cross-linked enzyme aggregates | Lactiplantibacillus plantarum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0074 | - |
N-acetyl-D-mannosamine | cross-linked enzyme aggregates, pH 7.5, 37°C | Lactiplantibacillus plantarum | |
0.03 | - |
N-acetyl-D-mannosamine | soluble enzyme, pH not specified in the publication, temperature not specified in the publication | Lactiplantibacillus plantarum | |
0.09 | - |
pyruvate | cross-linked enzyme aggregates, pH 7.5, 37°C | Lactiplantibacillus plantarum | |
0.11 | - |
pyruvate | soluble enzyme, pH not specified in the publication, temperature not specified in the publication | Lactiplantibacillus plantarum |