4.1.3.3: N-acetylneuraminate lyase

This is an abbreviated version!
For detailed information about N-acetylneuraminate lyase, go to the full flat file.

Reaction

Synonyms

Acetylneuraminate pyruvate-lyase, class I NAL, EcNanA, Lyase, acetylneuraminate, N-acetyl-d-neuraminic acid aldolase, N-acetyl-D-neuraminic acid lyase, N-Acetylneuraminate aldolase, N-Acetylneuraminate lyase, N-acetylneuraminate pyruvate lyase, N-Acetylneuraminate pyruvate-lyase, N-Acetylneuraminic acid aldolase, N-Acetylneuraminic acid lyase, N-Acetylneuraminic aldolase, N-Acetylneuraminic lyase, NAL, NALase, NanA, NANA lyase, Neu5Ac aldolase, Neu5Ac lyase, NeuAc aldolase, NeuAc lyase, Neuraminate aldolase, Neuraminic acid aldolase, Neuraminic aldolase, NPL, PmNanA, sialate aldolase, Sialate lyase, sialate-pyruvate lyase, Sialic acid aldolase, Sialic aldolase

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.3 Oxo-acid-lyases

                4.1.3.3 N-acetylneuraminate lyase

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Results	in table
4	Activating Compound
2595	AA Sequence
14	Application
1	CAS Registry Number
31	Cloned(Commentary)
12	Crystallization (Commentary)
16	Disease/ Diagnostics
4	Expression
6	General Information
3	General Stability
92	Inhibitors
57	kcat/KM [mM/s]
5	Ki Value [mM]
160	KM Value [mM]
3	Localization
14	Metals/Ions
36	Molecular Weight [Da]
34	Natural Substrates/ Products (Substrates)
132	Organism
3	Oxidation Stability
6	Pathway
213	PDB ID
35	pH Optimum
9	pH Range
6	pH Stability
3	pI Value
105	Protein Variants
37	Purification (Commentary)
8	Reaction
8	Reaction Type
91	Reference
1	Renatured (Commentary)
16	Source Tissue
29	Specific Activity [micromol/min/mg]
1	Storage Stability
186	Substrates and Products (Substrate)
31	Subunits
96	Synonyms
1	Systematic Name
31	Temperature Optimum [°C]
2	Temperature Range [°C]
32	Temperature Stability [°C]
111	Turnover Number [1/s]

Specific Activity

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Specific Activity on EC 4.1.3.3 - N-acetylneuraminate lyase

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.015

Escherichia coli

-

N-acetyl-D-mannosamine + pyruvate, 37°C

33337

0.0693

Sus scrofa

Q9BEC7

immobilized recombinant enzyme, the activity yield of immobilized enzyme is approximately 10% of the free enzyme

701855

0.14

Escherichia coli

-

crude cell extract, at 37°C

678004

0.42 - 1.5

Sus scrofa

-

-

650651

1.2 - 2.2

Escherichia coli

-

N-acetylneuraminic acid, 25°C

33336

1.5

Sus scrofa

-

-

651432

10

2 entries

11

Staphylococcus aureus

Q6GK01

purified recombinant enzyme, pH 8.0, temperature not specified in the publication

726631

11.36

Clostridium perfringens

-

-

651103

12.7

Staphylococcus carnosus

B9DIJ2

purified recombinant His-taggged enzyme, pH 7.0, 37°C

727656

14.6

Escherichia coli

-

after purification, at 37°C

678004

2.45

Escherichia coli

-

crude extract, in 50 mM potassium phosphate buffer, pH 7.5, at 37°C

701855

35.5

Escherichia coli

-

N-acetylneuraminic acid

33332

36.26

2 entries

38.7

Escherichia coli

-

N-acetylneuraminic acid

33324

4.33

Staphylococcus aureus

Q2G160

purified recombinant wild-type enzyme, pH 7.4, 30°C

727317

56.8

Escherichia coli

-

N-acetylneuraminic acid

33314

6

Staphylococcus aureus

Q2G160

purified recombinant refolded wild-type enzyme, pH 7.4, 30°C

727317

7.65

Lactiplantibacillus plantarum

-

pH 7.0, 37°C

713843

additional information

8 entries

10

Clostridium perfringens

-

-

650651

10

Escherichia coli

-

N-acetylneuraminic acid

33330

36.26

Escherichia coli

-

after 14.8fold purification, in 50 mM potassium phosphate buffer, pH 7.5, at 37°C

701855

36.26

Sus scrofa

Q9BEC7

purified enzyme after expression in Escherichia coli cells, enzyme activity is assayed in reaction solution (20 mM N-acetyl-D-neuraminic acid in 50 mM potassium phosphate buffer, pH 7.5)

701855

additional information

Bos taurus

-

-

33319

additional information

Clostridium perfringens

-

-

33316

additional information

Clostridium perfringens

-

-

33325

additional information

Escherichia coli

-

-

33314

additional information

Escherichia coli

-

after a 36 h incubation, the concentrations of N-acetyl-D-neuraminic acid from the reactions utilizing 0.4, 0.8, 1.2, 1.6, 2.0, and 2.2 M pyruvate as the carbon source are 7.5 (3.75%), 26.9 (3.45%), 43.9 (21.95%), 56.0 (28%), and 61.9 mM (30.95%), respectively. When using 2.2 M pyruvate, in the first 6 h, the concentration of N-acetyl-D-neuraminic acid reaches 29.5 mM (which is about half of the maximal concentration), and the productivity of N-acetyl-D-neuraminic acid is 1.6 g/l/h, the concentration of the intermediate N-acetyl-D-mannosamine also increases gradually from 0 to 64 mM during the process

701881

additional information

Haemophilus influenzae

-

-

33325

additional information

Sus scrofa

-

-

33321

additional information

Sus scrofa

Q9BEC7

cell density of Escherichia coli BL21 (DE3) carrying the pNA1 plasmid is 37 (OD600) after fermentation for 15 h, and the highest activity for N-acetyl-D-neuraminic acid lyase is 8.472 U/ml after fermentation for 9 h, enzyme activity is assayed in reaction solution (20 mM Neu5Ac in 50 mM potassium phosphate buffer, pH 7.5)

701855