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4.1.3.16: 4-Hydroxy-2-oxoglutarate aldolase

This is an abbreviated version!
For detailed information about 4-Hydroxy-2-oxoglutarate aldolase, go to the full flat file.

Word Map on EC 4.1.3.16

Reaction

4-hydroxy-2-oxoglutarate
=
pyruvate
+
glyoxylate

Synonyms

2-keto-4-hydroxybutyrate aldolase, 2-keto-4-hydroxyglutarate aldolase, 2-keto-4-hydroxyglutaric aldolase, 2-oxo-3-deoxy-6-phosphogluconate aldolase, 2-oxo-4-hydroxyglutarate aldolase, 2-oxo-4-hydroxyglutaric aldolase, 4-hydroxy-2-ketoglutarate aldolase, 4-hydroxy-2-ketoglutaric aldolase, 4-hydroxy-2-oxoglutarate aldolase, aldolase, 4-hydroxy-2-oxoglutarate, DHDPSL, dihydrodipicolinate synthase-like enzyme, DL-4-Hydroxy-2-ketoglutarate aldolase, EC 4.1.2.1, EC 4.1.2.31, eda, Entner-Doudoroff aldolase, hOGA, HOGA1, hydroxyketoglutarate aldolase, Hydroxyketoglutaric aldolase, KDPGlc aldolase, KHG-aldolase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.3 Oxo-acid-lyases
                4.1.3.16 4-Hydroxy-2-oxoglutarate aldolase

Engineering

Engineering on EC 4.1.3.16 - 4-Hydroxy-2-oxoglutarate aldolase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C257G
natural mutation associated with Primary Hyperoxaluria type 3. Mutant is quite unstable, has a tendency to aggregate, and retains no measurable activity
DeltaE315
natural mutation associated with Primary Hyperoxaluria type 3. Mutant is quite unstable, has a tendency to aggregate, and retains no measurable activity
G287V
K196A
complete loss of activity
N78A
20% of wild-type activity
N78Q
4% of wild-type activity
N78T
45% of wild-type activity
P190L
R255X
a truncation of 71 residues from the C-terminus associated with Primary Hyperoxaluria type 3. Mutant is quite unstable, has a tendency to aggregate, and retains no measurable activity
R303C
natural mutation associated with Primary Hyperoxaluria type 3. Mutant is quite unstable, has a tendency to aggregate, and retains no measurable activity
R70P
natural mutation associated with Primary Hyperoxaluria type 3. Mutant is quite unstable, has a tendency to aggregate, and retains no measurable activity
R97C
natural mutation associated with Primary Hyperoxaluria type 3. Mutant is quite unstable, has a tendency to aggregate, and retains no measurable activity
S198A
9% of wild-type activity
S198T
18% of wild-type activity
S77A
5% of wild-type activity
S77T
2% of wild-type activity
S77V
2% of wild-type activity
T280I
natural mutation associated with Primary Hyperoxaluria type 3. Mutant is quite unstable, has a tendency to aggregate, and retains no measurable activity
Y140F
122% of wild-type activity
Y168F
complete loss of activity
Y39X/R70X
naturally occuring mutations in a patient with type 3 atypical primary hyperoxaluria