4.1.2.55: 2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase

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For detailed information about 2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase, go to the full flat file.

Reaction

Synonyms

2-keto-3-deoxy-6-phosphogluconate aldolase, 2-keto-3-deoxygluconate aldolase, KD(P)G-aldolase, KDG aldolase, KDG-aldolase, KDGA, SacKdgA

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.2 Aldehyde-lyases

                4.1.2.55 2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase

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Results	in table
10	AA Sequence
1	Application
7	Cloned(Commentary)
5	Crystallization (Commentary)
2	General Information
3	Inhibitors
10	kcat/KM [mM/s]
1	Ki Value [mM]
28	KM Value [mM]
1	Metals/Ions
3	Molecular Weight [Da]
24	Natural Substrates/ Products (Substrates)
19	Organism
19	Pathway
24	PDB ID
8	pH Optimum
5	Protein Variants
8	Purification (Commentary)
4	Reaction
19	Reference
1	Specific Activity [micromol/min/mg]
85	Substrates and Products (Substrate)
3	Subunits
19	Synonyms
1	Systematic Name
13	Temperature Optimum [°C]
4	Temperature Range [°C]
6	Temperature Stability [°C]
24	Turnover Number [1/s]

Crystallization

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Crystallization on EC 4.1.2.55 - 2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

hanging drop diffusion method, three crystal forms: orthorhombic crystals of space group P2(1)2(1)2(1), which diffract to beyond 2.15 A, monoclinic crystals of space group C2, which diffract to 2.2 A, and cubic crystals of space group P4(2)32, which diffract to 3.4 A

Saccharolobus solfataricus

O54288

677304

in complex with 2-dehydro-3-deoxy-6-phospho-D-gluconate, vapor diffusion method

Saccharolobus solfataricus

O54288

747152

trapping of Schiff base complexes with natural substrates pyruvate, 2-dehydro-3-deoxy-D-gluconate, 2-dehydro-3-deoxy-D-galactonate and pyruvate plus D-glyceraldehyde at physiological pH by rapid soaking at low temperature followed by flash freezing prior to X-ray data collection. The complexes explain the substrate promiscuity of the enzyme with a rigid ligand-binding site able to accommodate both 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate

Saccharolobus solfataricus

O54288

665573

X-ray crystallographic analysis of the active site of the aldolase bound to pyruvate, 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate at 4°C, reveals that its catalytic mechanism is typical of the N-acetylneuraminic acid lyase superfamily

Saccharolobus solfataricus

O54288

715259

hanging-drop method, the enzyme crystallizes in two space groups, P3(1)21 and P6(5)22, for which diffraction data are obtained to a maximum resolution of 1.8 and 2.5 A

Sulfolobus acidocaldarius

Q4JC35

718771