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Literature summary for 4.1.2.55 extracted from

  • Wolterink-van Loo, S.; van Eerde, A.; Siemerink, M.A.; Akerboom, J.; Dijkstra, B.W.; van der Oost, J.
    Biochemical and structural exploration of the catalytic capacity of Sulfolobus KDG aldolases (2007), Biochem. J., 403, 421-430.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia Sulfolobus acidocaldarius
overexpression in Escherichia Sulfurisphaera tokodaii

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop method, the enzyme crystallizes in two space groups, P3(1)21 and P6(5)22, for which diffraction data are obtained to a maximum resolution of 1.8 and 2.5 A Sulfolobus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
pyruvate pH 6.0, 70°C Sulfurisphaera tokodaii
1.1
-
pyruvate pH 6.0, 70°C Sulfolobus acidocaldarius
3.9
-
D-glyceraldehyde pH 6.0, 70°C Sulfurisphaera tokodaii
6.3
-
D-glyceraldehyde pH 6.0, 70°C Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q4JC35
-
-
Sulfurisphaera tokodaii F9VPG1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfolobus acidocaldarius
-
Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows strict requirement for pyruvate Sulfolobus acidocaldarius ?
-
?
pyruvate + D-glyceraldehyde higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde. The enzyme forms a mixture of D-2-dehydro-3-deoxygluconate and D-2-dehydro-3-deoxy-galactonate Sulfurisphaera tokodaii 2-dehydro-3-deoxy-D-galactonate
-
r
pyruvate + D-glyceraldehyde higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde. The enzyme forms a mixture of D-2-keto-3-deoxygluconate and D-2-keto-3-deoxy-galactonate Sulfolobus acidocaldarius 2-dehydro-3-deoxy-D-galactonate
-
r
pyruvate + D-glyceraldehyde higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde. The enzyme forms a mixture of D-2-dehydro-3-deoxygluconate and D-2-dehydro-3-deoxy-galactonate Sulfurisphaera tokodaii 2-dehydro-3-deoxy-D-gluconate
-
r
pyruvate + D-glyceraldehyde higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde. The enzyme forms a mixture of D-2-keto-3-deoxygluconate and D-2-keto-3-deoxy-galactonate Sulfolobus acidocaldarius 2-dehydro-3-deoxy-D-gluconate
-
r
pyruvate + D-glyceraldehyde 3-phosphate higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde Sulfolobus acidocaldarius 2-dehydro-3-deoxy-D-galactonate 6-phosphate
-
r
pyruvate + D-glyceraldehyde 3-phosphate higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde Sulfolobus acidocaldarius 2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
r
pyruvate + glycolaldehyde
-
Sulfolobus acidocaldarius 2-dehydro-3-deoxy-D-xylonate
-
r
pyruvate + glycolaldehyde
-
Sulfolobus acidocaldarius 2-dehydro-3-deoxy-L-arabinonate
-
r
Pyruvate + glyoxylate
-
Sulfolobus acidocaldarius ?
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Sulfolobus acidocaldarius
70
-
assay at Sulfurisphaera tokodaii
90
-
-
Sulfurisphaera tokodaii
99
-
-
Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
t1/2: 110 min Sulfurisphaera tokodaii
100
-
t1/2: 18.9 min Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Sulfurisphaera tokodaii
6
-
assay at Sulfolobus acidocaldarius
6
-
assay at Sulfurisphaera tokodaii
6.5
-
-
Sulfolobus acidocaldarius