Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia | Sulfolobus acidocaldarius |
overexpression in Escherichia | Sulfurisphaera tokodaii |
Crystallization (Comment) | Organism |
---|---|
hanging-drop method, the enzyme crystallizes in two space groups, P3(1)21 and P6(5)22, for which diffraction data are obtained to a maximum resolution of 1.8 and 2.5 A | Sulfolobus acidocaldarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
pyruvate | pH 6.0, 70°C | Sulfurisphaera tokodaii | |
1.1 | - |
pyruvate | pH 6.0, 70°C | Sulfolobus acidocaldarius | |
3.9 | - |
D-glyceraldehyde | pH 6.0, 70°C | Sulfurisphaera tokodaii | |
6.3 | - |
D-glyceraldehyde | pH 6.0, 70°C | Sulfolobus acidocaldarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | Q4JC35 | - |
- |
Sulfurisphaera tokodaii | F9VPG1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus acidocaldarius |
- |
Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows strict requirement for pyruvate | Sulfolobus acidocaldarius | ? | - |
? | |
pyruvate + D-glyceraldehyde | higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde. The enzyme forms a mixture of D-2-dehydro-3-deoxygluconate and D-2-dehydro-3-deoxy-galactonate | Sulfurisphaera tokodaii | 2-dehydro-3-deoxy-D-galactonate | - |
r | |
pyruvate + D-glyceraldehyde | higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde. The enzyme forms a mixture of D-2-keto-3-deoxygluconate and D-2-keto-3-deoxy-galactonate | Sulfolobus acidocaldarius | 2-dehydro-3-deoxy-D-galactonate | - |
r | |
pyruvate + D-glyceraldehyde | higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde. The enzyme forms a mixture of D-2-dehydro-3-deoxygluconate and D-2-dehydro-3-deoxy-galactonate | Sulfurisphaera tokodaii | 2-dehydro-3-deoxy-D-gluconate | - |
r | |
pyruvate + D-glyceraldehyde | higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde. The enzyme forms a mixture of D-2-keto-3-deoxygluconate and D-2-keto-3-deoxy-galactonate | Sulfolobus acidocaldarius | 2-dehydro-3-deoxy-D-gluconate | - |
r | |
pyruvate + D-glyceraldehyde 3-phosphate | higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde | Sulfolobus acidocaldarius | 2-dehydro-3-deoxy-D-galactonate 6-phosphate | - |
r | |
pyruvate + D-glyceraldehyde 3-phosphate | higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde | Sulfolobus acidocaldarius | 2-dehydro-3-deoxy-D-gluconate 6-phosphate | - |
r | |
pyruvate + glycolaldehyde | - |
Sulfolobus acidocaldarius | 2-dehydro-3-deoxy-D-xylonate | - |
r | |
pyruvate + glycolaldehyde | - |
Sulfolobus acidocaldarius | 2-dehydro-3-deoxy-L-arabinonate | - |
r | |
Pyruvate + glyoxylate | - |
Sulfolobus acidocaldarius | ? | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Sulfolobus acidocaldarius |
70 | - |
assay at | Sulfurisphaera tokodaii |
90 | - |
- |
Sulfurisphaera tokodaii |
99 | - |
- |
Sulfolobus acidocaldarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
t1/2: 110 min | Sulfurisphaera tokodaii |
100 | - |
t1/2: 18.9 min | Sulfolobus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Sulfurisphaera tokodaii |
6 | - |
assay at | Sulfolobus acidocaldarius |
6 | - |
assay at | Sulfurisphaera tokodaii |
6.5 | - |
- |
Sulfolobus acidocaldarius |