Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
X-ray crystallographic analysis of the active site of the aldolase bound to pyruvate, 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate at 4°C, reveals that its catalytic mechanism is typical of the N-acetylneuraminic acid lyase superfamily | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
T157C | improved for 2-dehydro-3-deoxy-D-gluconate with a 75% diastereoisomeric ratio | Saccharolobus solfataricus |
T157C/Y132V | diastereoselective formation of 2-dehydro-3-deoxy-D-gluconate with much improved (91%) diastereoisomeric ratio | Saccharolobus solfataricus |
T157F/Y132V | diastereoselective formation of 2-dehydro-3-deoxy-D-gluconate with much improved (93%) diastereoisomeric ratio | Saccharolobus solfataricus |
T157V/A198L/D181Q | diastereoselective formation of 2-dehydro-3-deoxy-D-galactonate with much improved (88%) diastereoisomeric ratio | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
pyruvate | 70°C, pH is not specified in the publication, wild-type enzyme | Saccharolobus solfataricus | |
4.1 | - |
pyruvate | 70°C, pH is not specified in the publication, mutant enzyme T157F/Y132V | Saccharolobus solfataricus | |
5.3 | - |
D-glyceraldehyde | 70°C, pH is not specified in the publication, wild-type enzyme T157F/Y132V | Saccharolobus solfataricus | |
8 | - |
D-glyceraldehyde | 70°C, pH is not specified in the publication, mutant enzyme | Saccharolobus solfataricus | |
9.1 | - |
D-glyceraldehyde | 70°C, pH is not specified in the publication, mutant enzyme T157C/Y132V | Saccharolobus solfataricus | |
39.6 | - |
pyruvate | 70°C, pH is not specified in the publication, mutant enzyme T157C/Y132V | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | O54288 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + D-glyceraldehyde | the enzyme exhibits poor diastereocontrol in many of its aldol reactions, including the reaction of its natural substrates, pyruvate and D-glyceraldehyde, which afford a 55:45 mixture of D-2-keto-3-deoxygluconate and D-2-keto-3-deoxy-galactonate | Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-galactonate | - |
r | |
pyruvate + D-glyceraldehyde | the enzyme exhibits poor diastereocontrol in many of its aldol reactions, including the reaction of its natural substrates, pyruvate and D-glyceraldehyde, which afford a 55:45 mixture of D-2-keto-3-deoxygluconate and D-2-keto-3-deoxy-galactonate | Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-gluconate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
2-keto-3-deoxygluconate aldolase | - |
Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
D-glyceraldehyde | 70°C, pH is not specified in the publication, mutant enzyme | Saccharolobus solfataricus | |
0.07 | - |
pyruvate | 70°C, pH is not specified in the publication, mutant enzyme T157C/Y132V | Saccharolobus solfataricus | |
0.07 | - |
D-glyceraldehyde | 70°C, pH is not specified in the publication, mutant enzyme T157C/Y132V | Saccharolobus solfataricus | |
0.075 | - |
pyruvate | 70°C, pH is not specified in the publication, mutant enzyme T157F/Y132V | Saccharolobus solfataricus | |
4.6 | - |
pyruvate | 70°C, pH is not specified in the publication, wild-type enzyme | Saccharolobus solfataricus | |
5.5 | - |
D-glyceraldehyde | 70°C, pH is not specified in the publication, wild-type enzyme T157F/Y132V | Saccharolobus solfataricus |