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4.1.2.20: 2-dehydro-3-deoxyglucarate aldolase

This is an abbreviated version!
For detailed information about 2-dehydro-3-deoxyglucarate aldolase, go to the full flat file.

Word Map on EC 4.1.2.20

Reaction

2-dehydro-3-deoxy-D-glucarate
=
pyruvate
 +
2-hydroxy-3-oxopropanoate

Synonyms

2-dehydro-3-deoxygalactarate aldolase, 2-keto-3-deoxy gluconate aldolase, 2-keto-3-deoxy-glucarate aldolase, 2-keto-3-deoxyglucarate aldolase, 2-keto-3-deoxygluconate-specific aldolase, 4-hydroxy-2-ketoheptane-1,7-dioate aldolase, 5-KDGluc aldolase, 5-Keto-4-deoxy-(D)-glucarate aldolase, 5-keto-4-deoxy-D-glucarate aldolase, aldolase, 2-keto-3-deoxy-D-glucarate, DDG aldolase, HpcH, KDG aldolase, KDG-specific aldolase, KDGA, KDGLucA, YagE, YfaU

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.20 2-dehydro-3-deoxyglucarate aldolase

Crystallization

Crystallization on EC 4.1.2.20 - 2-dehydro-3-deoxyglucarate aldolase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
complexed with pyruvate
hanging drop vapor diffusion method
-
micro batch method, cocrystallization of YagE with 2-keto-3-deoxy galactonate and with pyruvate and glyceraldehyde in 100 mM HEPES pH 6.5, 200 mM MgCl2, 15% (w/v) PEG 3350, 50 mM pyruvate, and 50 mM glyceraldehyde
the crystal structure of apo-YfaU is determined to 1.39 A resolution and the structure of a Mg2+-pyruvate product complex to 1.93 A resolution
hanging drop vapour diffusion method with 10% PEG 8000, 100 mM Tris-HCl buffer pH 6.8 and 20 mM MnCl2
-