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Literature summary for 4.1.2.20 extracted from

  • Rea, D.; Hovington, R.; Rakus, J.F.; Gerlt, J.A.; Fueloep, V.; Bugg, T.D.; Roper, D.I.
    Crystal structure and functional assignment of YfaU, a metal ion dependent class II aldolase from Escherichia coli K12 (2008), Biochemistry, 47, 9955-9965.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the plasmid pT7 for expression in Escherichia coli B834DE3 pLacIRARE2 cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structure of apo-YfaU is determined to 1.39 A resolution and the structure of a Mg2+-pyruvate product complex to 1.93 A resolution Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
4-hydroxy-2-ketohexanoic acid data for Ni2+-activated YfaU Escherichia coli
0.078
-
2-dehydro-3-deoxy-L-rhamnonic acid data for Mg2+-activated YfaU Escherichia coli
0.1
-
4-hydroxy-2-ketoheptane-1,7-dioate data for Ni2+-activated YfaU Escherichia coli
0.1
-
4-hydroxy-2-ketopentanoic acid data for Ni2+-activated YfaU Escherichia coli
0.14
-
2-keto-3-deoxy-L-mannonate data for Mg2+-activated YfaU Escherichia coli
0.15
-
4-hydroxy-2-ketoheptane-1,7-dioate data for Mg2+-activated YfaU Escherichia coli
0.8
-
2-keto-3-deoxy-L-lyxonate data for Mg2+-activated YfaU Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Escherichia coli
Ni2+
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P23522
-
-

Purification (Commentary)

Purification (Comment) Organism
using metal affinity and size exclusion chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-L-rhamnonate
-
Escherichia coli ?
-
?
2-keto-3-deoxy-L-lyxonate
-
Escherichia coli ?
-
?
2-keto-3-deoxy-L-mannonate
-
Escherichia coli ?
-
?
4-hydroxy-2-ketoheptane-1,7-dioate
-
Escherichia coli pyruvate + succinic semialdehyde
-
?
4-hydroxy-2-ketohexanoic acid
-
Escherichia coli ?
-
?
4-hydroxy-2-ketopentanoic acid
-
Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
4-hydroxy-2-ketoheptane-1,7-dioate aldolase
-
Escherichia coli
HpcH
-
Escherichia coli
YfaU
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
2-keto-3-deoxy-L-lyxonate data for Mg2+-activated YfaU Escherichia coli
0.3
-
2-keto-3-deoxy-L-mannonate data for Mg2+-activated YfaU Escherichia coli
0.4
-
2-dehydro-3-deoxy-L-rhamnonic acid data for Mg2+-activated YfaU Escherichia coli
0.54
-
4-hydroxy-2-ketoheptane-1,7-dioate data for Mg2+-activated YfaU Escherichia coli
299
-
4-hydroxy-2-ketoheptane-1,7-dioate data for Ni2+-activated YfaU Escherichia coli
396
-
4-hydroxy-2-ketopentanoic acid data for Ni2+-activated YfaU Escherichia coli
447
-
4-hydroxy-2-ketohexanoic acid data for Ni2+-activated YfaU Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
activity assay Escherichia coli