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4.1.2.17: L-fuculose-phosphate aldolase

This is an abbreviated version!
For detailed information about L-fuculose-phosphate aldolase, go to the full flat file.

Word Map on EC 4.1.2.17

Reaction

L-Fuculose 1-phosphate
=
glycerone phosphate
+
(S)-lactaldehyde

Synonyms

aldolase, L-fuculose phosphate, F-1PA, Fuc-1PA, FucA, fuculose aldolase, fuculose-1-phosphate aldolase, L-Fuc1P aldolase, L-Fuculose 1-phosphate aldolase, L-Fuculose phosphate aldolase, L-Fuculose-1-P aldolase, L-fuculose-1-phosphate aldolase, L-fuculose-phosphate aldolase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.17 L-fuculose-phosphate aldolase

Engineering

Engineering on EC 4.1.2.17 - L-fuculose-phosphate aldolase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D120N
-
mutant with reduced specific activity
D76E
-
mutant with reduced specific activity
E142Q
-
mutant with increased specific activity
E73Q
no enzyme activity
E73Q/Y113F/Y209F
no enzyme activity
E73S
no enzyme activity
F131A
F131A/Del(207-215)
-
inactive
F131A/F206A
-
the relative activity of F131A/F206A towards L-fuculose-1-phosphate is approximately less than 0.5% as compared to the wild type enzyme
F131A/Y113A
-
inactive
F206A
-
the mutant has 22% retroaldol activity with L-fuculose-1-phosphate as compared to the wild type enzyme
H218N
-
mutant with reduced specific activity
H97N
-
mutant with reduced specific activity
K42M
-
mutant with reduced specific activity, can be inhibited by excess Zn2+
N28A
-
mutant with reduced specific activity
N29L
decreased kcat, increased Km
N29L/S71A
no enzyme activity
S71N
no enzyme activity
Y113A
-
the mutant has 0.5% retroaldol activity with L-fuculose-1-phosphate as compared to the wild type enzyme
D25L
-
negative effect on catalytic activity and substrate recognition
D25T
-
negative effect on catalytic activity and substrate recognition
additional information
-
the deletion mutants Del(211-215) and Del(207-215) show 11% and 2% retroaldol activity with L-fuculose-1-phosphate, respectively, as compared to the wild type enzyme