Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M-15 [pREP-4] cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F131A | the mutant has 1% retroaldol activity with L-fuculose-1-phosphate as compared to the wild type enzyme. The mutant uses (R)-N-benzyloxycarbonyl-prolinal as substrate and is highly stereoselective both with (R)-and with (S)-N-benzyloxycarbonyl-prolinal, exclusively producing the anti- and syn-aldol adducts, respectively | Escherichia coli |
F131A/Del(207-215) | inactive | Escherichia coli |
F131A/F206A | the relative activity of F131A/F206A towards L-fuculose-1-phosphate is approximately less than 0.5% as compared to the wild type enzyme | Escherichia coli |
F131A/Y113A | inactive | Escherichia coli |
F206A | the mutant has 22% retroaldol activity with L-fuculose-1-phosphate as compared to the wild type enzyme | Escherichia coli |
additional information | the deletion mutants Del(211-215) and Del(207-215) show 11% and 2% retroaldol activity with L-fuculose-1-phosphate, respectively, as compared to the wild type enzyme | Escherichia coli |
Y113A | the mutant has 0.5% retroaldol activity with L-fuculose-1-phosphate as compared to the wild type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,4-dideoxy-1,4-imino-L-arabinitol | - |
Escherichia coli | |
1,4-dideoxy-1,4-iminod-arabinitol | - |
Escherichia coli | |
D-fagomine | - |
Escherichia coli | |
L-fagomine | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone + (R)-N-benzyloxycarbonyl-alaninal | - |
Escherichia coli | 5-[[(benzyloxy)carbonyl]amino]-5,6-dideoxy-1-O-phosphonato-D-erythro-hex-2-ulose | - |
? | |
dihydroxyacetone + (S)-N-benzyloxycarbonyl-alaninal | - |
Escherichia coli | 5-[[(benzyloxy)carbonyl]amino]-5,6-dideoxy-1-O-phosphonato-D-erythro-hex-2-ulose | - |
? | |
dihydroxyacetone + benzyl (2R)-2-formylpyrrolidine-1-carboxylate | - |
Escherichia coli | (3R,4R)-4-[1-[(benzyloxy)carbonyl]pyrrolidin-2-yl]-3,4-dihydroxy-2-oxobutyl phosphate | - |
? | |
dihydroxyacetone + benzyl (2S)-2-formylpyrrolidine-1-carboxylate | - |
Escherichia coli | (3R,4S)-4-[1-[(benzyloxy)carbonyl]pyrrolidin-2-yl]-3,4-dihydroxy-2-oxobutyl phosphate | - |
? | |
dihydroxyacetone + benzyl [(2R)-3-methyl-1-oxobutan-2-yl]carbamate | - |
Escherichia coli | (3R,4R,5R)-5-[[(benzyloxy)carbonyl]amino]-3,4-dihydroxy-6-methyl-2-oxoheptyl phosphate | - |
? | |
dihydroxyacetone + benzyl [(2R)-4-methyl-1-oxopentan-2-yl]carbamate | - |
Escherichia coli | (3R,4R,5R)-5-[[(benzyloxy)carbonyl]amino]-3,4-dihydroxy-7-methyl-2-oxooctyl phosphate | - |
? | |
dihydroxyacetone + benzyl [(2S)-3-methyl-1-oxobutan-2-yl]carbamate | - |
Escherichia coli | (3R,4R,5R)-5-[[(benzyloxy)carbonyl]amino]-3,4-dihydroxy-6-methyl-2-oxoheptyl phosphate + (3R,4S,5R)-5-[[(benzyloxy)carbonyl]amino]-3,4-dihydroxy-6-methyl-2-oxoheptyl phosphate | 70:30 product ratio | ? | |
dihydroxyacetone + benzyl [(2S)-4-methyl-1-oxopentan-2-yl]carbamate | - |
Escherichia coli | (3R,4R,5R)-5-[[(benzyloxy)carbonyl]amino]-3,4-dihydroxy-7-methyl-2-oxooctyl phosphate | - |
? | |
dihydroxyacetone + benzyl [(3S)-3-methyl-1-oxopentan-2-yl]carbamate | - |
Escherichia coli | (3R,4R,5R,6S)-5-(((benzyloxy)carbonyl)amino)-3,4-dihydroxy-6-methyl-2-oxooctyl phosphate + (3R,4S,5R,6S)-5-(((benzyloxy)carbonyl)amino)-3,4-dihydroxy-6-methyl-2-oxooctyl phosphate | 78:22 product ratio | ? | |
L-fuculose 1-phosphate | - |
Escherichia coli | dihydroxyacetone phosphate + (S)-lactaldehyde | - |
? | |
additional information | (R)-N-benzyloxycarbonyl-prolinal is not a substrate for the wild type enzyme | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FucA | - |
Escherichia coli |
L-fuculose-1-phosphate aldolase | - |
Escherichia coli |