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Literature summary for 4.1.2.17 extracted from
- Structures of L-fuculose-1-phosphate aldolase mutants outlining motions during catalysis (2000), J. Mol. Biol., 303, 531-543.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E73Q | no enzyme activity | Escherichia coli |
| E73Q/Y113F/Y209F | no enzyme activity | Escherichia coli |
| E73S | no enzyme activity | Escherichia coli |
| N29L | decreased kcat, increased Km | Escherichia coli |
| N29L/S71A | no enzyme activity | Escherichia coli |
| S71N | no enzyme activity | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.2 | - |
L-Fuculose 1-phosphate | pH 7.5, 37°C, wild-type | Escherichia coli |  |
| 130 | - |
L-Fuculose 1-phosphate | pH 7.5, 37°C, N29Q mutant | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-fuculose 1-phosphate | Escherichia coli | - |
glycerone phosphate + (S)-lactaldehyde | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0AB87 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-fuculose 1-phosphate | - |
Escherichia coli | glycerone phosphate + (S)-lactaldehyde | - |
r | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.5 | - |
L-Fuculose 1-phosphate | pH 7.5, 37°C, N29Q mutant | Escherichia coli | |
| 19 | - |
L-Fuculose 1-phosphate | pH 7.5, 37°C, wild-type | Escherichia coli | |
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