4.1.1.81: threonine-phosphate decarboxylase

This is an abbreviated version!
For detailed information about threonine-phosphate decarboxylase, go to the full flat file.

Reaction

Synonyms

CobD, CobD gene product, L-Thr kinase/L-Thr-P decarboxylase, L-Thr kinase/L-Thr-phosphate decarboxylase, L-threonine-O-3-phosphate decarboxylase, MM2060, MmCobD, More, SMUL_1544

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.1 Carboxy-lyases

                4.1.1.81 threonine-phosphate decarboxylase

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Results	in table
9227	AA Sequence
1	CAS Registry Number
2	Cloned(Commentary)
2	Cofactor
2	Crystallization (Commentary)
12	General Information
1	KM Value [mM]
2	Metals/Ions
2	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
13	Organism
5	Pathway
6	Protein Variants
3	Purification (Commentary)
1	Reaction
10	Reference
19	Substrates and Products (Substrate)
2	Subunits
23	Synonyms
1	Systematic Name

Engineering

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Engineering on EC 4.1.1.81 - threonine-phosphate decarboxylase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

C434A

Methanosarcina mazei

Q8PVB2

site-directed mutagenesis, the MmCobDC434A variant has an ATPase activity that is comparable to wild-type despite having a growth phenotype similar to the DELTApduX vector control

760602

C458A

Methanosarcina mazei

Q8PVB2

site-directed mutagenesis, the MmCobDC458A variant has an ATPase activity that is comparable to wild-type despite having a growth phenotype similar to the DELTApduX vector control. The mutant variant has the lowest Fe to protein ratio

760602

K234A

Methanosarcina mazei

Q8PVB2

site-directed mutagenesis, the mutat variant lacks the ability to bind PLP effectively, resulting in 19 Fe per monomer, which is reduced compared to wild-type

760602

C434A

Methanosarcina mazei Goe1

-

site-directed mutagenesis, the MmCobDC434A variant has an ATPase activity that is comparable to wild-type despite having a growth phenotype similar to the DELTApduX vector control

-

C458A

Methanosarcina mazei Goe1

-

site-directed mutagenesis, the MmCobDC458A variant has an ATPase activity that is comparable to wild-type despite having a growth phenotype similar to the DELTApduX vector control. The mutant variant has the lowest Fe to protein ratio

-

K234A

Methanosarcina mazei Goe1

-

site-directed mutagenesis, the mutat variant lacks the ability to bind PLP effectively, resulting in 19 Fe per monomer, which is reduced compared to wild-type

-