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4.1.1.19: arginine decarboxylase

This is an abbreviated version!
For detailed information about arginine decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.19

Reaction

L-arginine
=
agmatine
 +
CO2

Synonyms

AaxB, AaxB protein, acid-induced biodegradative arginine decarboxylase, ADC, ADC 1, ADC 2, ADC1, ADC2, AdiA, ARGDC, arginine decarboxlase 2, arginine decarboxylase, arginine decarboxylase2, arginine:agmatine exchange system, ATADC1, AtADC2, AtDC2, bADC, Biosynthetic arginine decarboxylase, biosynthetic arginine decarboxylase 1, biosynthetic arginine decarboxylase 2, ClADC, CPn1032 homolog, dADC, Decarboxylase, arginine, HP0422, HVO_1958, L-Arginine decarboxylase, More, NtADC1, NtADC2, PaADC1, PaADC2, PBCV-1 DC, PdaD, PpADC, protein SSO0536, PtADC, PvlArgDC, pyruvoyl-dependent arginine decarboxylase, pyruvoyl-dependent arginine decraboxylase, slr0662, slr1312, SPE1, SPE2, SpeA, speA1, speA2, StADC, Synthetic arginine decarboxylase, Tk-PdaD, TK0149

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.19 arginine decarboxylase

Purification

Purification on EC 4.1.1.19 - arginine decarboxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2024fold after final purification step with MonoQ HR 5/5
ADC protein attached to the six-His tag at the C-terminal end is purified from parasite extracts by immobilized metal affinity chromatography through a Ni2+-agarose column
biosynthetic arginine decarboxylase and synthetic arginine decarboxylase
-
HisTrap column chromatography and Superdex 75 gel filtration
HiTrap Chelating HP column chromatography
Ni-NTA column chromatography, HiTrap Q column chromatography, and DEAE Sepharose column chromatography
-
Ni2+-affinity column chromatography and gel filtration
Ni2+-chelating affinity column chromatography and Superdex 200 gel filtration
-
nickel chelating column chromatography and Superdex 200 gel filtration
partially by subcellular fractionation
-
purification and cleavage of the N-terminal His-tag are performed
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration, to homogeneity
recombinant enzyme 12.4fold from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation and dialysis
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
recombinant His-tagged isozyme Adc1 from Escherichia coli by nickel affinity chromatography to homogeneity. Possible occurrence of N-terminal or C-terminal degradation of Adc1 during purification
recombinant His-tagged isozyme Adc2 from Escherichia coli by nickel affinity chromatography to homogeneity. Possible occurrence of N-terminal or C-terminal degradation of Adc2 during purification
recombinant His10-tagged enzyme from Escherichia coli strain Bl21(DE3) by nickel affinity chromatography, recombinant untagged enzyme from Escherichia coli strain Bl21(DE3) by anion exchange chromatography, most of the protein is found in the insoluble portion of cell lysate
-
recombinant His6-tagged enzyme from Escherichia coli strain SM10 by nickel affinity chromatography
recombinant protein using GST-tag
-
recombinant protein using His-tag