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4.1.1.19: arginine decarboxylase

This is an abbreviated version!
For detailed information about arginine decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.19

Reaction

L-arginine
=
agmatine
 +
CO2

Synonyms

AaxB, AaxB protein, acid-induced biodegradative arginine decarboxylase, ADC, ADC 1, ADC 2, ADC1, ADC2, AdiA, ARGDC, arginine decarboxlase 2, arginine decarboxylase, arginine decarboxylase2, arginine:agmatine exchange system, ATADC1, AtADC2, AtDC2, bADC, Biosynthetic arginine decarboxylase, biosynthetic arginine decarboxylase 1, biosynthetic arginine decarboxylase 2, ClADC, CPn1032 homolog, dADC, Decarboxylase, arginine, HP0422, HVO_1958, L-Arginine decarboxylase, More, NtADC1, NtADC2, PaADC1, PaADC2, PBCV-1 DC, PdaD, PpADC, protein SSO0536, PtADC, PvlArgDC, pyruvoyl-dependent arginine decarboxylase, pyruvoyl-dependent arginine decraboxylase, slr0662, slr1312, SPE1, SPE2, SpeA, speA1, speA2, StADC, Synthetic arginine decarboxylase, Tk-PdaD, TK0149

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.19 arginine decarboxylase

Cloned

Cloned on EC 4.1.1.19 - arginine decarboxylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
construction of negative mutant
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Escherichia coli strain MachI is used as a recipient for transformations during plasmid construction and for plasmid propagation and storage. Site-directed mutagenesis is performed on pPRDC.19. The mutant plasmids are transformed into Escherichia coli B834 (DE3) competent cells.
expressed as His-tag fusion protein in Escherichia coli BL21(DE3)pLysE
-
expressed in Arabidopsis thaliana
expressed in Arabidopsis thaliana ecotype Col-0
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expressed in Escherichia coli
expressed in Escherichia coli BL21 as GST-fusion protein
-
expressed in Escherichia coli BL21(DE3) cells
expressed in hairy roots of Lotus corniculatus
-
expressed in Oryza sativa
expressed in the T7 expression system as a cleavable poly-Histagged fusion construct in Escherichia coli strain Rosetta (DE3) pLysS
-
expressed in Trypanosoma cruzi, which lacks arginine decarboxylase in wild type
-
expression as His-tag fusion protein in Escherichia coli
Paramecium bursaria Chlorella virus-1
-
expression in COS-7 cells
expression in Escherichia coli
expression in Escherichia coli strain DE3
expression in Escherichia coli. The phylogeny of the crenarchaeal homologs suggests that the arginine decarboxylase gene evolves from a single duplication of an ancestral S-adenosylmethionine decarboxylase gene early in the crenarchaeota
expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
Paramecium bursaria chlorella virus
-
expression of sense and anti-sense constructs
expression of the enzyme in transformed hairy root lines in sense and antisense orientation, expression analysis
-
gene ADC, expression analysis under salt stress in calli, overview
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gene ADC, functional overexpression from retroviral vector hADC pLXSN in murine cortical astrocytes leading to and astrocyte-protective effect of human arginine decarboxylase transduction against oxygen-glucose deprivation (OGD), overview
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gene ADC, phylogenetic analysis and phylogeny reconstruction, overview
gene ADC, quantitative real-time PCR expression analysis
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gene ADC, quantitative real-time PCR expression analysis, recombinant expression of C-terminally YFP-tagged enzyme in Nicotiana benthamiana leaves via transformation method with Agrobacterium tumefaciens strain EHA105, coexpression with transcription factor ICE1 from Poncirus trifoliata
gene ADC, real-time RT-PCR enzyme expression analysis
-
gene ADC, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3)
gene ADC1, cloning from genomic DNA, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, expression analysis
gene ADC1, quantitative real-time PCR expression analysis
gene ADC1, quantitative real-time PCR isozyme expression analysis
gene ADC1, recombinant heterologous expression of GFP-tagged isozyme ADC1 as homodimers in Nicotiana benthamiana using the Agrobacterium tumefaciens GV3101/pMP90 strain for transformation. The green fluorescence of GFP-AtADC1 fusion is slightly present in the cytosol and it colocalized with the red auto-fluorescence of chlorophyll channel in Nicotiana benthamiana leaf epidermal cells
gene ADC1, semi-quantitative RT-PCR enzyme expression analysis, Adc1 shows constitutive expression, recombinant expression of His-tagged isozyme in Escherichia coli
gene ADC1, sequence comparisons
gene ADC2, quantitative real-time PCR expression analysis
gene ADC2, quantitative real-time PCR isozyme expression analysis
gene ADC2, recombinant expression as GFP-tagged protein in Arabidopsis thaliana, recombinant heterologous expression in Nicotiana benthamiana in using the Agrobacterium tumefaciens GV3101/pMP90 strain for transformation, the GFP-AtADC2 fluorescence occurs in the cytosol, and it colocalizes with the red autofluorescence of chlorophyll of tobacco leaf epidermal cells
gene ADC2, semi-quantitative RT-PCR enzyme expression analysis, Adc2 shows constitutive expression, recombinant expression of His-tagged isozyme in Escherichia coli
gene ADC2, sequence comparisons
gene adiA, expression of untagged and N-terminally His10-tagged wild-type enzyme and inactivated mutant in strain BW25113
gene adiA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene CPn1032, expression of untagged and N-terminally His10-tagged enzyme in Escherichia coli strain Bl21(DE3), CPn1032 expression complements the adiA null mutation in Escherichia coli strain DEG0121, which shows low arginine decarboxylase enzyme activity and is deficient in AdiC transporter
-
gene speA, expression of His-tagged enzyme in Escherichia coli
gene speA, recombinant expression of His6-tagged enzyme in Escherichia coli strain SM10
gene speA, recombinant overexpression of the enzyme in a soluble and active form in Escherichia coli strain BL21(DE3), method optimization leading to significant improvement of the production of agmatine. Method optimization, optimum conditions for agmatine production of the recombinant enzyme in vivo are 3.5 g/l intact cells, 4 mM Mg2+, 30 mM pyridoxal-5'-phosphate, pH 7, 37°C, method validation, overview. The specific activity of the overexpressing cell extract is 0.21 U/mg, which is 6.6fold higher than that of the BL21 cells not containing the overexpression plasmid
oat enzyme is expressed in Trypanosoma cruzi epimastigotes after transfection with a C-terminal six-His tag and as untagged enzyme, active ADC is expressed in the parasites and the primary translational product is cleaved into 2 polypeptides through a proteolytic process. When the his-tag is added to the C-terminal end of ADC it causes very significant changes on the metabolic stability and catalytic parameters of the heterologous enzyme expressed in the transformed parasites.
overexpression in Escherichia coli
-
profiles of the steady-state accumulation of Synechocystis ADC transcripts and ADC specific activities under various environmental conditions, overview
rapid amplification of cDNA ends (RACE) give rise to a full-length ADC cDNA (PpADC) with a complete open reading frame of 2178 bp, encoding a 725 amino acid polypeptide.
the hexahistidine tagged human ADC gene is delivered into mouse fibroblast cell line (NIH3T3) using retroviral vector and transfected into the PT-67 cell line