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3.6.4.B8: clamp-loader complex

This is an abbreviated version!
For detailed information about clamp-loader complex, go to the full flat file.

Word Map on EC 3.6.4.B8

Reaction

ATP
+
H2O
=
ADP
 +
phosphate

Synonyms

9-1-1 loader, ATP-dependent clamp loader complex, ATPase, cell cycle checkpoint protein, clamp loader, clamp loader complex, CLC, CTF18-RFC, ELG1-RFC, gamma clamp loader, gamma clamp loader complex, MacRFC complex, ORC, origin recognition complex, PCNA unloader, primary PCNA loader, RAD17, replication factor C, RF-C/activator 1 homolog, RFC, RFC clamp loader complex, RFC complex, RFC1, secondary PCNA loader, SsoRFC-complex

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.4 Acting on acid anhydrides to facilitate cellular and subcellular movement
                3.6.4.B8 clamp-loader complex

Cloned

Cloned on EC 3.6.4.B8 - clamp-loader complex

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of RFCS1Ma and RFCS2Ma as N-terminal His6-tagged proteins
Q8TSX5; Q8TUC8; Q8TPU4
gene holA gene encodes the N-terminal domain (residues 1-140) of the Escherichia coli gamma clamp loader, recombinant His-tagged mini-delta is obtained by expression in Escherichia coli strain BL21(DE3)
genes dnaX, and holA-D
recombinant expression of clamp loader complex subunits delta, gamma, delta', chi, and psi
recombinant expression of replication factor C subunits 2, 3, 4, and 5 with mutated truncated subunit RFC1
P35251; P35250; P40938; P35249; P40937
recombinant expression of small subunit RFCS, and full-length enzyme RFCSL in Escherichia coli strain Rosetta(DE3)/pLysS
the two SsoRFC polypeptides are coexpressed in Escherichia coli and purified as a complex. The small subunit is individually expressed in Escherichia coli and purified