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Literature summary for 3.6.4.B8 extracted from

  • Oakley, A.J.
    A structural view of bacterial DNA replication (2019), Protein Sci., 28, 990-1004 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.B8

Cloned(Commentary)

Cloned (Comment) Organism
genes dnaX, and holA-D Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Escherichia coli
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P28630 AND P28631 AND P06710 AND P28905 AND P28632 subunits delta, delta', gamma/tau, chi, and psi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Escherichia coli ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
heptamer the Escherichia coli clamp loader complex CLC comprises seven subunits: delta, tau_n, gamma(3-n), delta', psi, and chi. The delta and delta' subunits (encoded by holA and holB) together with three copies of gamma and/or tau (encoded by dnaX) form a heteropentamer. The chi and psi subunits (encoded by holC and holD) are not required for clamp-loading activity, but serve to bridge the CLC with single-stranded DNA (ssDNA)-binding protein (SSB). The gamma subunit is a truncated (residues 1-431) form of tau (residues 1-643) resulting from a programmed frameshift during translation of dnaX mRNA. Enzyme pentameric and heptameric complex structures with bound DNA or SSB and without, overview. The pentameric deltagamma3delta' complex in the apo, ADP-, or ATP-gammaS-bound states are nearly identical Escherichia coli
More clamp loader complex organization of tau domains, the truncated version gamma comprises domains I-III, overview Escherichia coli

Synonyms

Synonyms Comment Organism
ATP-dependent clamp loader complex
-
 Escherichia coli
clamp loader complex
-
 Escherichia coli
CLC
-
 Escherichia coli

General Information

General Information Comment Organism
additional information structure of Escherichia coli clamp loader complex CLC in complex with primed template DNA (PDB ID 3GLI). The chi and psi subunits serve to link the clamp loader complex and ssDNA binding protein SSB, with chi binding to SSB. Through its interaction with the CLC and SSB, the chi-psi complex plays an important role in the processivity of Okazaki fragment synthesis. Folds of chi and psi are similar to mononucleotide and dinucleotide binding proteins, respectively Escherichia coli
physiological function sliding clamps are actively loaded onto primed template DNA by ATP-dependent clamp loader complexes. The complex of chi and psi enzyme complex subunits plays an important role in the processivity of Okazaki fragment synthesis Escherichia coli