Information on EC 3.6.4.B8 - clamp-loader complex

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.6.4.B8
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
clamp-loader complex
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + H2O = ADP + phosphate
show the reaction diagram
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
O28219: Replication factor C small subunit, O29072: Replication factor C large subunit
O28219 and O29072
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O
ADP + phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O
ADP + phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DNA
Q9UXF5 and Q9UXF6
ATPase activity is activated by primed DNA templates, such as poly(dA)-oligo(dT). ATPase activity of the SsoRFC-complex is substantially stimulated by the primed homopolymer, whereas no effect is detected in the presence of poly(dA)400. The maximal activation (about tenfold) of the ATP-hydrolyzing activity is measured in the presence of poly(dA)4000-oligo(dT)45 at 140 nM
dsDNA
Q8TSX5 and Q8TUC8 and Q8TPU4
the MacRFC complex (a protein complex of Mac-RFCS1, MacRFCS2, and MacRFCL) possesses very low intrinsic ATPase activity. This activity is stimulated about 12fold by dsDNA
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Proliferating cell nuclear antigen
Q8TSX5 and Q8TUC8 and Q8TPU4
the MacRFC complex (a protein complex of Mac-RFCS1, MacRFCS2, and MacRFCL) possesses very low intrinsic ATPase activity. This activity is stimulated about 3fold by Methanosarcina acetivorans proliferating cell nuclear antigen
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singly primed single-stranded DNA
Q8TSX5 and Q8TUC8 and Q8TPU4
the MacRFC complex (a protein complex of Mac-RFCS1, MacRFCS2, and MacRFCL) possesses very low intrinsic ATPase activity. This activity is stimulated about 58fold by singly primed single-stranded DNA
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ssDNA
Q8TSX5 and Q8TUC8 and Q8TPU4
the MacRFC complex (a protein complex of Mac-RFCS1, MacRFCS2, and MacRFCL) possesses very low intrinsic ATPase activity. This activity is stimulated about 33fold by ssDNA
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.025
ATP
O28219 and O29072
wild-type enzyme, in the presence of DNA and Proliferating-Cell-Nuclear-Antigen (PCNA), pH and temperature not specified in the publication
additional information
ATP
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.7
ATP
Archaeoglobus fulgidus
O28219 and O29072
wild-type enzyme, in the presence of DNA and Proliferating-Cell-Nuclear-Antigen (PCNA), pH and temperature not specified in the publication
additional information
ATP
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
Q8TSX5 and Q8TUC8 and Q8TPU4
1 * 67000 (MacRFCL) + 1 * 38000 (MacRFCS2) + 1 * 35000 (MacRFCS1), SDS-PAGE of His6-tagged subunits
37200
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1 * 58100, subunit RFC-L, + 4 * 37200, subunit RFC-S, SDS-PAGE
37773
Q9UXF5 and Q9UXF6
4 * 37773 + 1 * 46787, calculated from sequence
46000
Q9UXF5 and Q9UXF6
4 * 38000 + 1 * 46000, the homo-tetramer of the small subunit is complexed with one large subunit, SDS-PAGE
46787
Q9UXF5 and Q9UXF6
4 * 37773 + 1 * 46787, calculated from sequence
58100
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1 * 58100, subunit RFC-L, + 4 * 37200, subunit RFC-S, SDS-PAGE
67000
Q8TSX5 and Q8TUC8 and Q8TPU4
1 * 67000 (MacRFCL) + 1 * 38000 (MacRFCS2) + 1 * 35000 (MacRFCS1), SDS-PAGE of His6-tagged subunits
200000
Q9UXF5 and Q9UXF6
gel filtration
203000
-
light scattering
208000
-
gel filtration
344500
Q8TSX5 and Q8TUC8 and Q8TPU4
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heteropentamer
oligomer
pentamer
trimer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structure of replication factor C domains 1 and 2 at 2.1 A, crystal structure of replication factor C ADPNP complex at 3.5 A and at 4.0 A, hanging drop vapour diffusion method
O28219 and O29072
electron microscopic structure of an archaeal RFC–(proliferating cell nuclear antigen)–DNA complex at 12 A resolution
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
O28219 and O29072
the two SsoRFC polypeptides are coexpressed in Escherichia coli and purified as a complex. The small subunit is individually expressed in Escherichia coli and purified
Q9UXF5 and Q9UXF6
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of RFCS1Ma and RFCS2Ma as N-terminal His6-tagged proteins
Q8TSX5 and Q8TUC8 and Q8TPU4
recombinant expression of small subunit RFCS, and full-length enzyme RFCSL in Escherichia coli strain Rosetta(DE3)/pLysS; recombinant expression of small subunit RFCS, and full-length enzyme RFCSL in Escherichia coli strain Rosetta(DE3)/pLysS
the two SsoRFC polypeptides are coexpressed in Escherichia coli and purified as a complex. The small subunit is individually expressed in Escherichia coli and purified
Q9UXF5 and Q9UXF6
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R84A/R90A/T120A/K149A
additional information