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Literature summary for 3.6.4.B8 extracted from
- Biochemical characterization of a clamp-loader complex homologous to eukaryotic replication factor C from the hyperthermophilic archaeon Sulfolobus solfataricus (2000), J. Mol. Biol., 301, 61-73.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DNA | ATPase activity is activated by primed DNA templates, such as poly(dA)-oligo(dT). ATPase activity of the SsoRFC-complex is substantially stimulated by the primed homopolymer, whereas no effect is detected in the presence of poly(dA)400. The maximal activation (about tenfold) of the ATP-hydrolyzing activity is measured in the presence of poly(dA)4000-oligo(dT)45 at 140 nM | Saccharolobus solfataricus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the two SsoRFC polypeptides are coexpressed in Escherichia coli and purified as a complex. The small subunit is individually expressed in Escherichia coli and purified | Saccharolobus solfataricus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37773 | - |
4 * 37773 + 1 * 46787, calculated from sequence | Saccharolobus solfataricus |
| 38000 | - |
4 * 38000 + 1 * 46000, the homo-tetramer of the small subunit is complexed with one large subunit, SDS-PAGE | Saccharolobus solfataricus |
| 46000 | - |
4 * 38000 + 1 * 46000, the homo-tetramer of the small subunit is complexed with one large subunit, SDS-PAGE | Saccharolobus solfataricus |
| 46787 | - |
4 * 37773 + 1 * 46787, calculated from sequence | Saccharolobus solfataricus |
| 200000 | - |
gel filtration | Saccharolobus solfataricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Saccharolobus solfataricus | - |
ADP + phosphate | - |
? | |
| ATP + H2O | Saccharolobus solfataricus P2 | - |
ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q9UXF5 and Q9UXF6 | Q9UXF5: Replication factor C small subunit, Q9UXF6: Replication factor C large subunit | - |
| Saccharolobus solfataricus P2 | Q9UXF5 and Q9UXF6 | Q9UXF5: Replication factor C small subunit, Q9UXF6: Replication factor C large subunit | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| the two SsoRFC polypeptides are coexpressed in Escherichia coli and purified as a complex. The small subunit is individually expressed in Escherichia coli and purified | Saccharolobus solfataricus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + H2O = ADP + phosphate | multisubunit clamp loader complexes use ATP to open sliding clamp rings and place them around the 3' end of primer-template junctions | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Saccharolobus solfataricus | ADP + phosphate | - |
? | |
| ATP + H2O | ATPase activity is activated by primed DNA templates, such as poly(dA)-oligo(dT). The SsoRFC-complex binds poly(dA)-oligo(dT), but not the unprimed homopolymer | Saccharolobus solfataricus | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Saccharolobus solfataricus P2 | ADP + phosphate | - |
? | |
| ATP + H2O | ATPase activity is activated by primed DNA templates, such as poly(dA)-oligo(dT). The SsoRFC-complex binds poly(dA)-oligo(dT), but not the unprimed homopolymer | Saccharolobus solfataricus P2 | ADP + phosphate | - |
? | |
| additional information | the enzyme stimulates the synthetic activity of Sulfolobus solfataricus B1-type DNA polymerase in reactions containing primed M13mp18 DNA, ATP, and either of the two poliferating cell nuclear antigen-like processivity factors of Sulfolobus solfataricus (039p and 048p) | Saccharolobus solfataricus | ? | - |
? | |
| additional information | the enzyme stimulates the synthetic activity of Sulfolobus solfataricus B1-type DNA polymerase in reactions containing primed M13mp18 DNA, ATP, and either of the two poliferating cell nuclear antigen-like processivity factors of Sulfolobus solfataricus (039p and 048p) | Saccharolobus solfataricus P2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heteropentamer | 4 * 37773 + 1 * 46787, calculated from sequence | Saccharolobus solfataricus |
| heteropentamer | 4 * 38000 + 1 * 46000, the homo-tetramer of the small subunit is complexed with one large subunit, SDS-PAGE | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SsoRFC-complex | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
assay at | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Saccharolobus solfataricus |
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