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3.5.3.3: creatinase

This is an abbreviated version!
For detailed information about creatinase, go to the full flat file.

Word Map on EC 3.5.3.3

Reaction

Creatine
+
H2O
=
sarcosine
 +
Urea

Synonyms

CAH, Creatine amidinohydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.3 In linear amidines
                3.5.3.3 creatinase

Temperature Stability

Temperature Stability on EC 3.5.3.3 - creatinase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 50
-
purified enzyme, stable
26 - 43
thermal stability of the bioelectrode studied by measuring the current at different temperatures ranging from 26 to 43°C in the presence of creatine at 150 microM and a phosphate buffer (50 mM, pH 7.0)
30
-
irreversible loss of some of the activity above
40
-
stable
40 - 50
the enzyme is stable below 40°C but completely loses its activity after 30 min preincubation at 50°C
55
-
15 min, completely destroyed
80 - 90
the activities of creatinase in wild-type and osw2D spores are more stable than those of free creatinase at higher temperatures. Such thermostability is not observed for creatinase in dit1D spores