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3.5.3.3: creatinase

This is an abbreviated version!
For detailed information about creatinase, go to the full flat file.

Word Map on EC 3.5.3.3

Reaction

Creatine
+
H2O
=
sarcosine
 +
Urea

Synonyms

CAH, Creatine amidinohydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.3 In linear amidines
                3.5.3.3 creatinase

General Stability

General Stability on EC 3.5.3.3 - creatinase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
addition of 0.2 M NaCl stabilizes against the solubilizing effect of urea. Methylurea has higher denaturing capacity than urea. At glycerol concentrations up to 20% creatinase is in its native state
-
enzyme shows anomalously low intrinsic stability and is inaccessible to reconstitution after preceding deactivation, denaturation and dissociation
-
stability of creatine amidinohydrolase-containing polyurethane hydrogels stored in buffer at 37°C decreases after 1 week, PEGylation of creatine-amidinohydrolase leads to decrease of half-life when stored in buffer at 37°C
-
stabilized by addition of nonionic detergents with a hydrophile-lipophile balance value of more than 14, chelating agents, or preservatives
-
unused enzyme in the immobilized creatininase-creatinase-sarcosine oxidase enzyme system maintains activity for at least 6 months. Activity remains unchanged after heavy daily use of 20-30 days
-
upon increasing the concentration of the enzyme from 0.003 to 0.024 mg/ml the apparent concentration of guanidinium chloride where 50% denaturation are observed shifts from 0.35 to 0.44 M
-