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Literature summary for 3.5.3.3 extracted from
- Effects of pH, temperature and reaction products on the performance of an immobilized creatininase-creatinase-sarcosine oxidase enzyme system for creatinine determination (1992), Anal. Chim. Acta, 268, 331-345.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| unused enzyme in the immobilized creatininase-creatinase-sarcosine oxidase enzyme system maintains activity for at least 6 months. Activity remains unchanged after heavy daily use of 20-30 days | Flavobacterium sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| sarcosine | - |
Flavobacterium sp. |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Flavobacterium sp. | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| creatine + H2O | - |
Flavobacterium sp. | sarcosine + urea | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
immobilized creatininase-creatinase-sarcosine oxidase enzyme system | Flavobacterium sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
irreversible loss of some of the activity above | Flavobacterium sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
immobilized creatininase-creatinase-sarcosine oxidase enzyme system | Flavobacterium sp. |
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