3.5.3.3: creatinase

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For detailed information about creatinase, go to the full flat file.

CAH, Creatine amidinohydrolase

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Results	in table
1	Activating Compound
448	AA Sequence
11	Application
1	CAS Registry Number
7	Cloned(Commentary)
5	Crystallization (Commentary)
2	General Information
6	General Stability
45	Inhibitors
17	KM Value [mM]
3	Localization
4	Metals/Ions
18	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
6	Organic Solvent Stability
47	Organism
6	Pathway
4	PDB ID
13	pH Optimum
5	pH Range
6	pH Stability
5	Protein Variants
9	Purification (Commentary)
2	Reaction
1	Reaction Type
44	Reference
1	Renatured (Commentary)
2	Source Tissue
9	Specific Activity [micromol/min/mg]
46	Substrates and Products (Substrate)
15	Subunits
10	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
4	Temperature Range [°C]
9	Temperature Stability [°C]
1	Turnover Number [1/s]

Purification on EC 3.5.3.3 - creatinase

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ammonium sulfate precipitation, HiPrep column chromatography, and Superdex 200 gel filtration

native enzyme 10.17fold to homogeneity by anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration