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3.5.1.97: acyl-homoserine-lactone acylase

This is an abbreviated version!
For detailed information about acyl-homoserine-lactone acylase, go to the full flat file.

Word Map on EC 3.5.1.97

Reaction

an N-acyl-L-homoserine lactone
+
H2O
=
L-homoserine lactone
 +
a carboxylate

Synonyms

AAC, acyl-homoserine lactone acylase, acyl-HSL acylase, acylhomoserine-lactone acylase, AHL acylase, AHL amidase, AHL-acylase, AhlM, AIBP, AiiC, AiiD, AiiO, AmiE, APTM01, AtPVA, auto-inducer inhibitor from Cyanobacteria, FaGL1422, HacA, HacB, KcPGA, long-chain AHL acylase, MacQ, More, N-acyl-homoserine lactone acylase, N-acylhomoserine lactone acylase, N-acylhomoserine lactone-acylase, PA0305, PA2385, PA2385 protein, PaPVA, pfmA, PvdQ, PvdQ acylase, QqaR, QuiP, quorum quenching acylhomoserine lactone acylase, quorum quenching enzyme, quorum-quenching AHL acylase, quorum-quenching enzyme, quorum-quenching N-acyl homoserine lactone acylase PvdQ, quorum-quenching N-acylhomoserine lactone acylase, SlPVA

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.97 acyl-homoserine-lactone acylase

Cloned

Cloned on EC 3.5.1.97 - acyl-homoserine-lactone acylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned and expressed in Escherichia coli DH10B
-
cloned and overexpressed
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DH5alpha cells
-
expressed in Escherichia coli Origami cells
-
expression in Escherichia coli
expression in Escherichia coli strain BL21 and in Vibrio anguillarum strain TB0008
expression in Escherichia coli strain DH10B
-
gene aiiD, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain DH5alpha, expression of AiiD in Pseudomonas aeruginosa strain PAO1 without showing catalytic function, but reducing the ability of the bacterium to exhibit quorum-regulated swarming motility, the bacterium is attenuated in nematode killing
-
gene aiiO, DNA and amino acid sequence determination and analysis, sequence comparisons, complementation of the aiiO::Tn5 mutation with the wild-type aiiO gene, expressionin Escherichia coli strain BL21(DE3)
gene amiE, from genomic library, DNA and amino acid sequence determination and analysis, phylogenetic tree, sequence comparisons, amiE is transferred by a putative transposon, cloning in Escherichia coli strain DHalpha, recombinant expression in Pseudomonas aeruginosa strain PAO1 leading to reduced N-acyl-L-homoserine lactone accumulation and elastase activity
gene macQ, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic and analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain Origami 2(DE3). Recombinant plasmid (pMQ28) confers 2 to 16fold higher MIC values toward all six beta-lactam antibiotics in Escherichia coli Origami 2(DE3) than the strain with a control vector. This indicates that MacQ confers multiple beta-lactam antibiotic resistance on a host strain by hydrolyzing beta-lactam antibiotics
-
gene pfmA, sequence comparisons, cloning of PfmA and recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene pvdQ, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, genetic structure analysis, recombinant expression in Escherichia coli strain BL21
phylogenetic analysis of isolate VM4 within the genus Delftia
-