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Literature summary for 3.5.1.97 extracted from

  • Lin, Y.H.; Xu, J.L.; Hu, J.; Wang, L.H.; Ong, S.L.; Leadbetter, J.R.; Zhang, L.H.
    Acyl-homoserine lactone acylase from Ralstonia strain XJ12B represents a novel and potent class of quorum-quenching enzymes (2003), Mol. Microbiol., 47, 849-860.
    View publication on PubMed
Search for more literature for 3.5.1.97

Application

Application Comment Organism
biotechnology the enzyme can be used in quenching quorum sensing Ralstonia sp.

Cloned(Commentary)

Cloned (Comment) Organism
gene aiiD, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain DH5alpha, expression of AiiD in Pseudomonas aeruginosa strain PAO1 without showing catalytic function, but reducing the ability of the bacterium to exhibit quorum-regulated swarming motility, the bacterium is attenuated in nematode killing Ralstonia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acyl-(S)-homoserine lactone + H2O Ralstonia sp. inactivation of N-acylhomoserine lactone signaling in regulation of infection and virulence functions (S)-homoserine lactone + carboxylic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Ralstonia sp.
-
 gene aiiD, isolated from a mixed-species biofilm
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with penicillin Ralstonia sp. ?
-
 ?
N-3-oxodecanoyl-(S)-homoserine lactone + H2O hydrolysis of the amide bond Ralstonia sp. (S)-homoserine lactone + 3-oxodecanoic acid
-
 ?
N-acyl-(S)-homoserine lactone + H2O inactivation of N-acylhomoserine lactone signaling in regulation of infection and virulence functions Ralstonia sp. (S)-homoserine lactone + carboxylic acid
-
 ?

Synonyms

Synonyms Comment Organism
acyl-homoserine lactone acylase
-
 Ralstonia sp.
AHL-acylase
-
 Ralstonia sp.
AiiD
-
 Ralstonia sp.
More the enzyme belongs to the quorum-quenching enzymes Ralstonia sp.
quorum-quenching enzyme
-
 Ralstonia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Ralstonia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Ralstonia sp.