Cloned (Comment) | Organism |
---|---|
gene amiE, from genomic library, DNA and amino acid sequence determination and analysis, phylogenetic tree, sequence comparisons, amiE is transferred by a putative transposon, cloning in Escherichia coli strain DHalpha, recombinant expression in Pseudomonas aeruginosa strain PAO1 leading to reduced N-acyl-L-homoserine lactone accumulation and elastase activity | Acinetobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Acinetobacter sp. | the enzyme shows a high level of degrading activity against N-acyl-L-homoserine lactones with long acyl chains but no activity against N-acyl-L-homoserine lactones with acyl chains shorter than eight carbons | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | A0A077JIE7 | activated-sludge isolate, gene amiE | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows a high level of degrading activity against N-acyl-L-homoserine lactones with long acyl chains but no activity against N-acyl-L-homoserine lactones with acyl chains shorter than eight carbons | Acinetobacter sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AHL acylase | - |
Acinetobacter sp. |
AmiE | - |
Acinetobacter sp. |
long-chain AHL acylase | - |
Acinetobacter sp. |
N-acylhomoserine lactone acylase | - |
Acinetobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Acinetobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Acinetobacter sp. |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the amidase family. AmiE shows homology with a member of the amidases (EC 3.5.1.4) but not with any known AHL acylase enzymes. An amino acid sequence of AmiE from Ooi24 shows greater than 99% identities with uncharacterized proteins from Acinetobacter ursingii CIP 107286 and Acinetobacter sp. strain CIP 102129, but it is not found in the draft or complete genome sequences of other Acinetobacter strains. Phylogenetic relationship between AmiE and known AHL acylases and phylogenetic tree | Acinetobacter sp. |
physiological function | the enzyme hydrolyzes the amide bond of N-acyl-L-homoserine lactones, quorum-sensing signal molecules. The recombinant enzyme in Pseudomonas aeruginosa PAO1 reduces N-acyl-L-homoserine lactone accumulation and elastase activity, which are regulated by AHL-mediated quorum sensing | Acinetobacter sp. |