3.5.1.78: Glutathionylspermidine amidase
This is an abbreviated version!
For detailed information about Glutathionylspermidine amidase, go to the full flat file.
Word Map on EC 3.5.1.78
-
3.5.1.78
-
trypanosomatids
-
gsh
-
leishmania
-
polyamine
-
histidine-dependent
-
fasciculata
-
gamma-glu-cys-gly
-
synthetases
-
tetrahedral
-
n1,n8-bisglutathionylspermidine
-
crithidia
-
kinetoplastida
-
s-thiolation
-
anti-parasitic
-
thiol-redox
-
druggable
-
neglected
-
sulfenic
-
slow-binding
-
tripeptide
-
phosphonate
-
protozoa
-
ligases
- 3.5.1.78
-
trypanosomatids
- gsh
- leishmania
- polyamine
-
histidine-dependent
- fasciculata
- gamma-glu-cys-gly
- synthetases
-
tetrahedral
-
n1,n8-bisglutathionylspermidine
-
crithidia
- kinetoplastida
-
s-thiolation
-
anti-parasitic
-
thiol-redox
-
druggable
-
neglected
-
sulfenic
-
slow-binding
- tripeptide
- phosphonate
-
protozoa
- ligases
Reaction
Synonyms
Amidase, glutathionylspermidine, Glutathionylspermidine amidohydrolase (spermidine-forming), Glutathionylspermidine amidohydrolase [spermidine-forming], Glutathionylspermidine synthetase/amidase, GSP amidase, Gsp synthetase/amidase, GspA, GspSA, GSS, trypanothione synthetase-amidase
ECTree
3.5.1.78 Glutathionylspermidine amidaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.5.1.78 - Glutathionylspermidine amidase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
glutathionylspermidine disulfide + H2O
glutathione + ?
a coupled reaction of Gsp amidase and glutathione reductase
products of the coupled reaction of Gsp amidase and glutathione reductase: spermidine, glutathionylspermidine-glutathione mixed disulfide, and glutathione disulfide are generated
-
?
glutathionylspermidine + H2O
glutathione + spermidine
-
GspSA catalyzes the formation of a peptide bond between GSH and spermidine, cf. EC 6.3.1.8
-
r
glutathionylspermidine + H2O
glutathione + spermidine
-
-
GspSA catalyzes the formation of a peptide bond between GSH and spermidine, cf. EC 6.3.1.8
-
r
glutathionylspermidine + H2O
glutathione + spermidine
the N-terminal domain catalyzes the hydrolysis of the conjugate
GspSA catalyzes the formation of a peptide bond between GSH and spermidine, cf. EC 6.3.1.8
-
r
trypanothione + H2O
glutathione + glutathionylspermidine
-
hydrolysis at 1.5% the rate of glutathionylspermidine
-
?
additional information
?
-
bifunctional enzyme that catalyzes the ATP-dependent formation and hydrolysis of glutathionylspermidine, a conjugate of glutathione and spermidine. Proteins YgiC and YjfC, encoded by genes ygiC and yjfC, show ATPase activity, but are not glutathionylspermidine synthetases
-
-
?
additional information
?
-
-
bifunctional enzyme that catalyzes the ATP-dependent formation and hydrolysis of glutathionylspermidine, a conjugate of glutathione and spermidine. Proteins YgiC and YjfC, encoded by genes ygiC and yjfC, show ATPase activity, but are not glutathionylspermidine synthetases
-
-
?
additional information
?
-
glutathionylspermidine S-thiolated peptides and proteins are tested as substrates. The protein substrates are treated with disulfide or biotinated glutathionylspermidine to generate biotin-labeled glutathionylspermidine S-thiolated proteins for the detection with an anti-biotin antibody after the reaction with the GspSA. A variety of glutathionylspermidine-derivatized substrates are hydrolyzed yielding spermidine and glutathione S-thiolated protein/peptides
-
-
?
