Literature summary for 3.5.1.78 extracted from

Bollinger, J.M.; Kwon, D.S.; Huisman, G.W.; Kolter, R.; Walsh, C.T.
Glutathionylspermidine metabolism in Escherichia coli. Purification, cloning, overproduction, and characterization of a bifunctional glutathionylspermidine synthetase/amidase (1995), J. Biol. Chem., 270, 14031-14041.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli

General Stability

General Stability	Organism
the presence of Mg2+, ATP and reduced glutathione, with or without spermidine, markedly protects the enzyme from cleavage by trypsin	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.9	-	glutathionylspermidine	-	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70000	-	or trimer, 2 or 3 * 70000, SDS-PAGE	Escherichia coli
70000	-	or dimer, 3 or 2 * 70000, SDS-PAGE	Escherichia coli
170000	-	gel filtration, the deviation of the estimated molecular mass from 140000 of a dimer might result from the dimer having a non-globular shape	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	bifunctional enzyme glutathionylspermidine synthetase/amidase	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glutathionylspermidine + H2O	-	Escherichia coli	glutathione + spermidine	-	?

Subunits

Subunits	Comment	Organism
dimer	or trimer, 2 or 3 * 70000, SDS-PAGE	Escherichia coli
trimer	or dimer, 3 or 2 * 70000, SDS-PAGE	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.1	-	glutathionylspermidine	-	Escherichia coli

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Release 2023.1 (January 2023)