Literature summary for 3.5.1.78 extracted from

Chiang, B.Y.; Chen, T.C.; Pai, C.H.; Chou, C.C.; Chen, H.H.; Ko, T.P.; Hsu, W.H.; Chang, C.Y.; Wu, W.F.; Wang, A.H.; Lin, C.H.
Protein S-thiolation by Glutathionylspermidine (Gsp): the role of Escherichia coli Gsp synthetase/amidase in redox regulation (2010), J. Biol. Chem., 285, 25345-25353.

Search for more literature for 3.5.1.78

Inhibitors

Inhibitors	Comment	Organism	Structure
H2O2	50% inhibition with 0.25 mM. Inactivation is above 95% with 0.5 mM H2O2 for 5 min. Cysteine thiol of the GspSA amidase active-site nucleophile Cys59 is transiently inactivated by H2O2 oxidation to sulfenic acid which leads to an accumulation of glutathionylspermidine and an increased level of glutathionylspermidine S-thiolated proteins after oxidative stress. The hypersensitivities of GspSA and GspSA/glutaredoxin null mutants to H2O2 support the idea that GspSA and glutaredoxin act synergistically to regulate the redox environment of Escherichia coli	Escherichia coli K-12

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli K-12	P0AES0	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
H2O2-mediated inactivation of the amidase activity can be recovered by the addition of 4 mM glutathione. ca. 80% of the enzyme activity are rescued	Escherichia coli K-12

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glutathionylspermidine + H2O	-	Escherichia coli K-12	glutathione + spermidine	-	?
glutathionylspermidine disulfide + H2O	a coupled reaction of Gsp amidase and glutathione reductase	Escherichia coli K-12	glutathione + ?	products of the coupled reaction of Gsp amidase and glutathione reductase: spermidine, glutathionylspermidine-glutathione mixed disulfide, and glutathione disulfide are generated	?
additional information	glutathionylspermidine S-thiolated peptides and proteins are tested as substrates. The protein substrates are treated with disulfide or biotinated glutathionylspermidine to generate biotin-labeled glutathionylspermidine S-thiolated proteins for the detection with an anti-biotin antibody after the reaction with the GspSA. A variety of glutathionylspermidine-derivatized substrates are hydrolyzed yielding spermidine and glutathione S-thiolated protein/peptides	Escherichia coli K-12	?	-	?

Synonyms

Synonyms	Comment	Organism
GSP amidase	-	Escherichia coli K-12
Gsp synthetase/amidase	bifunctional enzyme, EC 3.5.1.78 and EC 6.3.1.8	Escherichia coli K-12
GspSA	-	Escherichia coli K-12

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Release 2023.1 (January 2023)