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3.5.1.61: mimosinase

This is an abbreviated version!
For detailed information about mimosinase, go to the full flat file.

Word Map on EC 3.5.1.61

Reaction

(S)-2-amino-3-(3-hydroxy-4-oxo-4H-pyridin-1-yl)propanoate
+
H2O
=
3-hydroxy-4H-pyrid-4-one
 +
L-serine

Synonyms

mimosine degrading enzyme, mimosine-degrading enzyme, Mp mimosinase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.61 mimosinase

Cloned

Cloned on EC 3.5.1.61 - mimosinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene seq3, DNA and amino acid sequence determination and analysis, codon optimization and recombinant expression in Escherichia coli
generation of a synthetic gene, cloning method overview, subcloning in Escherichia coli strain JM109, sequence comparison with wild-type gene, recombinant enzyme expression of the synthetic gene syn-mimosinase ORF in Escherichia coli strain BL21(DE3) pLysS