Information on EC 3.5.1.61 - mimosinase

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The expected taxonomic range for this enzyme is: Leucaena leucocephala

EC NUMBER
COMMENTARY hide
3.5.1.61
-
RECOMMENDED NAME
GeneOntology No.
mimosinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-2-amino-3-(3-hydroxy-4-oxo-4H-pyridin-1-yl)propanoate + H2O = 3-hydroxy-4H-pyrid-4-one + L-serine
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amides
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
mimosine amidohydrolase
An enzyme from Leucaena leucocephala leaf, which also contains the toxic amino acid, mimosine.
CAS REGISTRY NUMBER
COMMENTARY hide
104118-49-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the carbon-nitrogen lyase catalyzes the first step of mimosine degradation. Mimosine is a toxic nonprotein aromatic amino acid
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-2-amino-3-(3-hydroxy-4-oxo-4H-pyridin-1-yl)propanoate + H2O
3-hydroxy-4(1H)-pyrid-4-one + L-serine
show the reaction diagram
(S)-2-amino-3-(3-hydroxy-4-oxo-4H-pyridin-1-yl)propanoate + H2O
3-hydroxy-4H-pyrid-4-one + pyruvate + NH3
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-2-amino-3-(3-hydroxy-4-oxo-4H-pyridin-1-yl)propanoate + H2O
3-hydroxy-4H-pyrid-4-one + pyruvate + NH3
show the reaction diagram
A8CEI3
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
dependent on, enzyme-bound
additional information
no requirement for an 2oxo acid
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
hydroxylamine
complete inhibition at 0.05 mM, reversible by 100 nM pyridoxal 5'-phosphate
tyrosine
-
competitive inhibitor, 0.14 mM: 90% of maximal activity, 0.28 mM: 80% of maximal activity, 0.42 mM: 78% of maximal activity, 0.56 mM: 72% of maximal activity, 0.96 mM: 56% of maximal activity, 1 mM: 50% of maximal activity
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.64
beta-[N-(3-hydroxy-4(1H)-pyridone)]-alpha-aminopropionic acid
-
mimosine
-
additional information
additional information
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reaction kinetics
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 12
high catalytic activities at pH 7.5-10.0, inactive below pH 7.0
additional information
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22 - 45
enzyme catalytic activity sharply decreases at 45C or higher and below 22C
35 - 65
-
appreciable activity between
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
the enzyme has a chloroplast transit peptide
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
x * 45000 recombinant enzyme, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 45000 recombinant enzyme, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
purified recombinant enzyme, pH 8.5, 37C, around 80% activity remaining, fairly stable up to 40C
50
purified recombinant enzyme, pH 8.5, 37C, the enzyme activity shows a sharp reduction
70
-
partial denaturation of the enzyme at
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme from Escherichia coli
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene seq3, DNA and amino acid sequence determination and analysis, codon optimization and recombinant expression in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nutrition
-
implication for reducing the toxicity of Leucaena used for stock feed, the product 3-hydroxy-4(1H)-pyrid-4-one, DHP, is goitrogenic in animals but is less toxic than mimosine, conversion of mimosine to DHP is therefore beneficial for the use of Leucaena as animal feed