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Literature summary for 3.5.1.61 extracted from

  • Tangendjaja, B.; Lowry, J.B.; Wills, R.B.H.
    Isolation of a mimosine degrading enzyme from Leucaena leaf (1986), J. Sci. Food Agric., 37, 523-526.
No PubMed abstract available
Search for more literature for 3.5.1.61

Inhibitors

Inhibitors Comment Organism Structure
additional information 3-hydroxy-4(1H)-pyridone, DHP, 0.4-3.2 mM, no effect, no feedback inhibitor Leucaena leucocephala
tyrosine competitive inhibitor, 0.14 mM: 90% of maximal activity, 0.28 mM: 80% of maximal activity, 0.42 mM: 78% of maximal activity, 0.56 mM: 72% of maximal activity, 0.96 mM: 56% of maximal activity, 1 mM: 50% of maximal activity Leucaena leucocephala

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information reaction kinetics Leucaena leucocephala
1.64
-
 beta-[N-(3-hydroxy-4(1H)-pyridone)]-alpha-aminopropionic acid mimosine Leucaena leucocephala

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 PAGE Leucaena leucocephala

Organism

Organism UniProt Comment Textmining
Leucaena leucocephala
-
 tropical legume
-

Purification (Commentary)

Purification (Comment) Organism
partial Leucaena leucocephala

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Leucaena leucocephala
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-amino-3-(3-hydroxy-4-oxo-4H-pyridin-1-yl)propanoate + H2O free toxic amino acid mimosine, 3-(3-hydroxy-4-oxo-1(4H)-pyridyl)-L-alanine, beta-[N-(3-hydroxy-4(1H)-pyridone)]-alpha-aminopropionic acid, beta-[N-(3-hydroxy-4-oxypyridyl)]-alpha-aminopropionic acid Leucaena leucocephala 3-hydroxy-4(1H)-pyrid-4-one + L-serine DHP, less toxic than mimosine ?
beta-[N-(3-hydroxy-4(1H)-pyridone)]-alpha-aminopropionic acid + H2O
-
 Leucaena leucocephala ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Leucaena leucocephala

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Leucaena leucocephala