3.5.1.49: formamidase
This is an abbreviated version!
For detailed information about formamidase, go to the full flat file.
Word Map on EC 3.5.1.49
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3.5.1.49
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kynurenine
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urease
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2,3-dioxygenase
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n-formyl-l-kynurenine
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2-aminofluorene
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methylotrophus
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methylophilus
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n-formylation
- 3.5.1.49
- kynurenine
- urease
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2,3-dioxygenase
- n-formyl-l-kynurenine
- 2-aminofluorene
- methylotrophus
- methylophilus
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n-formylation
Reaction
Synonyms
acetamidase/formamidase, Amds/fmds, AmiF, AmiF formamidase, BceAmiF, Fmd, formamidase, formamidase AmiF, Formamide amidohydrolase, formamide aminohydrolase, HpyAmiF, TE19
ECTree
3.5.1.49 formamidaseAdvanced search results
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results (Engineering
Engineering on EC 3.5.1.49 - formamidase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
E140D
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the mutation does not significantly alter enzyme folding and shows no detectable activity
Y191F
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the mutation does not significantly alter enzyme folding and shows 11.8% activity compared to the wild type enzyme
E140D
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the mutation does not significantly alter enzyme folding and shows no detectable activity
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W136H
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this mutation causes the absence of overexpression of the enzyme
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Y191F
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the mutation does not significantly alter enzyme folding and shows 11.8% activity compared to the wild type enzyme
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SeMet-substituted form
MAD data collection
fur mutant
nikR fur double mutant
nikR mutant
fur mutant
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amidase activity at pH 5.5: wild-type 0.6, mutant 3.0 - at pH 7.0: wild-type 0.1, mutant 2.9
fur mutant
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formamidase activity at pH 5.5: wild-type 25, mutant 41 - at pH 7.0: wild-type 5, mutant 41
fur mutant
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urease activity at pH 5.5: wild-type 20, mutant 23 - at pH 7.0: wild-type 4, mutant 3
nikR fur double mutant
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amidase activity at pH 5.5: wild-type 0.6, mutant 1.9 - at pH 7.0: wild-type 0.1, mutant 3.0
nikR fur double mutant
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formamidase activity at pH 5.5: wild-type 25, mutant 30 - at pH 7.0: wild-type 5, mutant 40
nikR fur double mutant
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urease activity at pH 5.5: wild-type 20, mutant 14 - at pH 7.0: wild-type 4, mutant 6
nikR mutant
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amidase activity at pH 5.5: wild-type 0.6, mutant 0.2 - at pH 7.0: wild-type 0.1, mutant 0.1
nikR mutant
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formamidase activity at pH 5.5: wild-type 25, mutant 8 - at pH 7.0: wild-type 5, mutant 5
nikR mutant
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urease activity at pH 5.5: wild-type 20, mutant 13 - at pH 7.0: wild-type 4, mutant 5
