3.5.1.49: formamidase
This is an abbreviated version!
For detailed information about formamidase, go to the full flat file.
Word Map on EC 3.5.1.49
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3.5.1.49
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kynurenine
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urease
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2,3-dioxygenase
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n-formyl-l-kynurenine
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2-aminofluorene
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methylotrophus
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methylophilus
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n-formylation
- 3.5.1.49
- kynurenine
- urease
-
2,3-dioxygenase
- n-formyl-l-kynurenine
- 2-aminofluorene
- methylotrophus
- methylophilus
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n-formylation
Reaction
Synonyms
acetamidase/formamidase, Amds/fmds, AmiF, AmiF formamidase, BceAmiF, Fmd, formamidase, formamidase AmiF, Formamide amidohydrolase, formamide aminohydrolase, HpyAmiF, TE19
ECTree
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Purification
Purification on EC 3.5.1.49 - formamidase
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expressed in the soluble form by Escherichia coli XL1 Blue MRF cells (5mg fusion protein per liter culture), purified on a Glutathione Sepharose 4B Resin column, cleaved with thrombin
glutatione Sepharose column chromatography, DEAE Sepharose column chromatography, and phenyl Sepharose column chromatography
Ni-NTA His-binding affinity chromatography, gel filtration on a Sephacryl S-200 column
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration
Talon metal-affinity resin column chromatography and Superdex 200 gel filtration
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