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Literature summary for 3.5.1.49 extracted from

  • van Vliet, A.H.; Kuipers, E.J.; Stoof, J.; Poppelaars, S.W.; Kusters, J.G.
    Acid-responsive gene induction of ammonia-producing enzymes in Helicobacter pylori is mediated via a metal-responsive repressor cascade (2004), Infect. Immun., 72, 766-773.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.49

Activating Compound

Activating Compound Comment Organism Structure
Ni2+
-
 Helicobacter pylori

Protein Variants

Protein Variants Comment Organism
fur mutant amidase activity at pH 5.5: wild-type 0.6, mutant 3.0 - at pH 7.0: wild-type 0.1, mutant 2.9 Helicobacter pylori
fur mutant formamidase activity at pH 5.5: wild-type 25, mutant 41 - at pH 7.0: wild-type 5, mutant 41 Helicobacter pylori
fur mutant urease activity at pH 5.5: wild-type 20, mutant 23 - at pH 7.0: wild-type 4, mutant 3 Helicobacter pylori
nikR fur double mutant amidase activity at pH 5.5: wild-type 0.6, mutant 1.9 - at pH 7.0: wild-type 0.1, mutant 3.0 Helicobacter pylori
nikR fur double mutant formamidase activity at pH 5.5: wild-type 25, mutant 30 - at pH 7.0: wild-type 5, mutant 40 Helicobacter pylori
nikR fur double mutant urease activity at pH 5.5: wild-type 20, mutant 14 - at pH 7.0: wild-type 4, mutant 6 Helicobacter pylori
nikR mutant amidase activity at pH 5.5: wild-type 0.6, mutant 0.2 - at pH 7.0: wild-type 0.1, mutant 0.1 Helicobacter pylori
nikR mutant formamidase activity at pH 5.5: wild-type 25, mutant 8 - at pH 7.0: wild-type 5, mutant 5 Helicobacter pylori
nikR mutant urease activity at pH 5.5: wild-type 20, mutant 13 - at pH 7.0: wild-type 4, mutant 5 Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formamide + H2O Helicobacter pylori
-
 formate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.05
-
 amidase activity wild-type Helicobacter pylori 26695, pH 7.0, 0 micromol NiCl2 supplementation Helicobacter pylori
0.05
-
 amidase activity wild-type Helicobacter pylori 26695, pH 7.0, 1 micromol NiCl2 supplementation Helicobacter pylori
0.1
-
 amidase activity wild-type Helicobacter pylori 26695, pH 7.0 Helicobacter pylori
0.1
-
 amidase activity wild-type Helicobacter pylori 26695, pH 7.0, 100 micromol NiCl2 supplementation Helicobacter pylori
0.5
-
 amidase activity wild-type Helicobacter pylori 26695, pH 7.0, 500 micromol NiCl2 supplementation Helicobacter pylori
0.55
-
 amidase activity wild-type Helicobacter pylori 26695, pH 7.0, 1000 micromol NiCl2 supplementation Helicobacter pylori
0.6
-
 amidase activity wild-type Helicobacter pylori 26695, pH 5.5 Helicobacter pylori
5
-
 formamidase activity wild-type Helicobacter pylori 26695, pH 7.0 Helicobacter pylori
5
-
 urease activity wild-type Helicobacter pylori 26695, pH 7.0 Helicobacter pylori
5.2
-
 formamidase activity wild-type Helicobacter pylori 26695, pH 7.0, 1000 micromol NiCl2 supplementation Helicobacter pylori
6
-
 formamidase activity wild-type Helicobacter pylori 26695, pH 7.0, 0 micromol NiCl2 supplementation Helicobacter pylori
6
-
 formamidase activity wild-type Helicobacter pylori 26695, pH 7.0, 1 micromol NiCl2 supplementation Helicobacter pylori
8
-
 formamidase activity wild-type Helicobacter pylori 26695, pH 7.0, 500 micromol NiCl2 supplementation Helicobacter pylori
8
-
 urease activity wild-type Helicobacter pylori 26695, pH 7.0, 0 micromol NiCl2 supplementation Helicobacter pylori
8.1
-
 formamidase activity wild-type Helicobacter pylori 26695, pH 7.0, 100 micromol NiCl2 supplementation Helicobacter pylori
20
-
 urease activity wild-type Helicobacter pylori 26695, pH 5.5 Helicobacter pylori
25
-
 formamidase activity wild-type Helicobacter pylori 26695, pH 5.5 Helicobacter pylori
30
-
 urease activity wild-type Helicobacter pylori 26695, pH 7.0, 1 micromol NiCl2 supplementation Helicobacter pylori
60
-
 urease activity wild-type Helicobacter pylori 26695, pH 7.0, 100 micromol NiCl2 supplementation Helicobacter pylori
70
-
 urease activity wild-type Helicobacter pylori 26695, pH 7.0, 1000 micromol NiCl2 supplementation Helicobacter pylori
75
-
 urease activity wild-type Helicobacter pylori 26695, pH 7.0, 500 micromol NiCl2 supplementation Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formamide + H2O
-
 Helicobacter pylori formate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
formamidase
-
 Helicobacter pylori