3.5.1.119: Pup amidohydrolase
This is an abbreviated version!
For detailed information about Pup amidohydrolase, go to the full flat file.
Word Map on EC 3.5.1.119
-
3.5.1.119
-
ubiquitin-like
-
tuberculosis
-
mycobacterial
-
depupylation
-
actinobacteria
-
isopeptide
-
deamidated
-
ligases
-
pup-proteasome
-
atpase
-
transacylase
-
malonyl
-
doom
-
adp
-
medicine
-
pharmacology
-
drug development
-
analysis
- 3.5.1.119
-
ubiquitin-like
- tuberculosis
-
mycobacterial
-
depupylation
- actinobacteria
-
isopeptide
-
deamidated
- ligases
-
pup-proteasome
- atpase
-
transacylase
-
malonyl
-
doom
- adp
- medicine
- pharmacology
- drug development
- analysis
Reaction
Synonyms
deamidase of Pup, deamidase/depupylase, deamidase/depupylase Dop, depupylase, depupylase Dop, depupylase/deamidase, Dop, DPUP, Pup deamidase, Rv2112, Rv2112c
ECTree
3.5.1.119 Pup amidohydrolaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.5.1.119 - Pup amidohydrolase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[His6-myo-inositol-1-phosphate synthetase]-L-lysine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + [His6-myo-inositol-1-phosphate synthetase]-L-lysine
N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[malonyl Co-A acyl carrier protein transacylase]-L-lysine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + [malonyl Co-A acyl carrier protein transacylase]-L-lysine
N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine
[prokaryotic ubiquitin-like protein]-fluorescein-5-carboxamide-L-lysine + H2O
[prokaryotic ubiquitin-like protein]-L-lysine + 5-carboxyfluorescein
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + NH3
N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[His6-myo-inositol-1-phosphate synthetase]-L-lysine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + [His6-myo-inositol-1-phosphate synthetase]-L-lysine
-
-
-
?
N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[His6-myo-inositol-1-phosphate synthetase]-L-lysine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + [His6-myo-inositol-1-phosphate synthetase]-L-lysine
-
-
-
?
N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[malonyl Co-A acyl carrier protein transacylase]-L-lysine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + [malonyl Co-A acyl carrier protein transacylase]-L-lysine
-
-
-
?
N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[malonyl Co-A acyl carrier protein transacylase]-L-lysine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + [malonyl Co-A acyl carrier protein transacylase]-L-lysine
-
-
-
?
N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine
the enzyme catalyzes the nucleophilic attack of water on the terminal carbonyl atom of Pup, releasing ammonium in the case of deamidation or the substrate lysine in case of depupylation
-
-
?
N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine
the enzyme catalyzes the nucleophilic attack of water on the terminal carbonyl atom of Pup, releasing ammonium in the case of deamidation or the substrate lysine in case of depupylation
-
-
?
[prokaryotic ubiquitin-like protein]-fluorescein-5-carboxamide-L-lysine + H2O
[prokaryotic ubiquitin-like protein]-L-lysine + 5-carboxyfluorescein
hydrolysis of the isopeptide bond in Pup-Fl and release the fluorescein-5-carboxamide lysine moiety
-
-
?
[prokaryotic ubiquitin-like protein]-fluorescein-5-carboxamide-L-lysine + H2O
[prokaryotic ubiquitin-like protein]-L-lysine + 5-carboxyfluorescein
hydrolysis of the isopeptide bond in Pup-Fl and release the fluorescein-5-carboxamide lysine moiety
-
-
?
[prokaryotic ubiquitin-like protein]-fluorescein-5-carboxamide-L-lysine + H2O
[prokaryotic ubiquitin-like protein]-L-lysine + 5-carboxyfluorescein
hydrolysis of the isopeptide bond in Pup-Fl and release the fluorescein-5-carboxamide lysine moiety
-
-
?
[prokaryotic ubiquitin-like protein]-fluorescein-5-carboxamide-L-lysine + H2O
[prokaryotic ubiquitin-like protein]-L-lysine + 5-carboxyfluorescein
-
hydrolysis of the isopeptide bond in Pup-Fl and release the fluorescein-5-carboxamide lysine moiety
-
-
?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + NH3
-
-
-
?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + NH3
deamidase (Dop) renders prokaryotic ubiquitin-like protein Pup competent for conjugation to substrates, which is then carried out by the Pup-ligase PafA. Pupylation is a signal for proteasomal degradation in bacteria
-
-
?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + NH3
the enzyme deamidates prokaryotic ubiquitin-like protein (Pup) on the C-terminal glutamine, thereby rendering Pup competent for conjugation
-
-
?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + NH3
the enzyme deamidates the carboxyl-terminal glutamine of Pup to glutamate, preparing it for ligation to target proteins by proteasome accessory factor A (PafA)
-
-
?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + NH3
the deamidase activity (Dop) renders prokaryotic ubiquitin-like protein Pup competent for conjugation to substrates, which is then carried out by the Pup-ligase PafA. The enzyme catalyzes the nucleophilic attack of water on the terminal carbonyl atom of Pup, releasing ammonium in the case of deamidation or the substrate lysine in case of depupylation
-
-
?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + NH3
the enzyme deamidates the carboxyl-terminal glutamine of Pup to glutamate
-
-
?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + NH3
deamidase (Dop) renders prokaryotic ubiquitin-like protein Pup competent for conjugation to substrates, which is then carried out by the Pup-ligase PafA. Pupylation is a signal for proteasomal degradation in bacteria
-
-
?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + NH3
the deamidase activity (Dop) renders prokaryotic ubiquitin-like protein Pup competent for conjugation to substrates, which is then carried out by the Pup-ligase PafA. The enzyme catalyzes the nucleophilic attack of water on the terminal carbonyl atom of Pup, releasing ammonium in the case of deamidation or the substrate lysine in case of depupylation
-
-
?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + NH3
the enzyme deamidates the carboxyl-terminal glutamine of Pup to glutamate, preparing it for ligation to target proteins by proteasome accessory factor A (PafA)
-
-
?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + NH3
the enzyme deamidates the carboxyl-terminal glutamine of Pup to glutamate
-
-
?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + NH3
-
-
-
?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + NH3
the enzyme deamidates prokaryotic ubiquitin-like protein (Pup) on the C-terminal glutamine, thereby rendering Pup competent for conjugation
-
-
?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + NH3
-
-
-
?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + NH3
-
-
-
?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
[prokaryotic ubiquitin-like protein]-L-glutamate + NH3
-
-
-
?
additional information
?
-
the enzyme is unable to hydrolyze linear Pup-substrate fusions
-
-
?
additional information
?
-
-
the enzyme is unable to hydrolyze linear Pup-substrate fusions
-
-
?
additional information
?
-
enzyme acts as a depupylase in the Pup proteasome system in vivo and in vitro. Dop removes prokaryotic ubiquitin-like protein Pup from substrates by specific cleavage of the isopeptide bond
-
-
?
additional information
?
-
the enzyme is unable to hydrolyze linear Pup-substrate fusions
-
-
?
additional information
?
-
enzyme acts as a depupylase in the Pup proteasome system in vivo and in vitro. Dop removes prokaryotic ubiquitin-like protein Pup from substrates by specific cleavage of the isopeptide bond
-
-
?
additional information
?
-
steric hindrance in positioning the isopeptide bond in the active site of Dop might be responsible for the observed differences in depupylation rather than differences in the overall affinity of pupylated substrate for Dop
-
-
?
additional information
?
-
steric hindrance in positioning the isopeptide bond in the active site of Dop might be responsible for the observed differences in depupylation rather than differences in the overall affinity of pupylated substrate for Dop
-
-
?
