Cloned (Comment) | Organism |
---|---|
the dop gene is cloned with a C-terminal His6-tag, expression in Escherichia coli | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O | Mycobacterium tuberculosis | the enzyme deamidates prokaryotic ubiquitin-like protein (Pup) on the C-terminal glutamine, thereby rendering Pup competent for conjugation | [prokaryotic ubiquitin-like protein]-L-glutamate + NH3 | - |
? | |
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O | Mycobacterium tuberculosis ATCC 25618 | the enzyme deamidates prokaryotic ubiquitin-like protein (Pup) on the C-terminal glutamine, thereby rendering Pup competent for conjugation | [prokaryotic ubiquitin-like protein]-L-glutamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WNU9 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | P9WNU9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme expressed in Escherichia coli | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O | - |
Mycobacterium tuberculosis | [prokaryotic ubiquitin-like protein]-L-glutamate + NH3 | - |
? | |
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O | the enzyme deamidates prokaryotic ubiquitin-like protein (Pup) on the C-terminal glutamine, thereby rendering Pup competent for conjugation | Mycobacterium tuberculosis | [prokaryotic ubiquitin-like protein]-L-glutamate + NH3 | - |
? | |
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O | - |
Mycobacterium tuberculosis ATCC 25618 | [prokaryotic ubiquitin-like protein]-L-glutamate + NH3 | - |
? | |
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O | the enzyme deamidates prokaryotic ubiquitin-like protein (Pup) on the C-terminal glutamine, thereby rendering Pup competent for conjugation | Mycobacterium tuberculosis ATCC 25618 | [prokaryotic ubiquitin-like protein]-L-glutamate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dop | - |
Mycobacterium tuberculosis |
Rv2112 | locus name | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | deamidation requires ATP as a cofactor but not its hydrolysis | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in pupylation | Mycobacterium tuberculosis |